Novel Bilobe Components in Trypanosoma brucei Identified Using Proximity-Dependent Biotinylation

Biotinylation by Myc-BirA*-TbMORN1 in vivo. Myc-BirA*-TbMORN1 cells were incubated with excess biotin in the presence or absence of 10 ng/ml tetracycline (Tet). (A and B) Immunofluorescence assay of intact cells labeled with anti-Myc antibodies and fluorescently conjugated streptavidin. In the presence of tetracycline (A), the two labels strongly overlapped. The bilobe (arrows, area enlarged in insets) was clearly visible. In the absence of tetracycline (B), only endogenously biotinylated proteins were labeled as a background signal. (C and D) Immunofluorescence assay of detergent-extracted cells labeled as in panels A and B. In the presence of tetracycline (C), colocalization between the anti-Myc and streptavidin labels was seen at the bilobe (arrows, area enlarged in insets). In the absence of tetracycline (D), no labeling was observed in either channel. Images are maximum intensity z-projections; overlap was confirmed in single 0.1-μm sections. Identical exposure times and channel level settings were used in image acquisition and processing. Scale bars, 2 μm. (E) Immunoblot of whole-cell lysates using HRP-conjugated streptavidin. In the presence of tetracycline, many additional polypeptides are strongly biotinylated. Equal loading was confirmed by blotting the samples with anti-TbBILBO1 (lower panel).

Purification of candidate TbMORN1 binding partners and near neighbors. (A) Schematic of purification protocol. Following incubation in excess biotin and the presence or absence of tetracycline (+/− Tet), the cells are incubated with 0.5% NP-40 (E1) and separated by centrifugation into detergent-soluble (S1) and detergent-insoluble (P1) fractions. The detergent-insoluble fraction is then further extracted with 0.4% SDS. Fractions S1 and S2 are incubated with streptavidin-conjugated paramagnetic beads, and the free unbound fractions (F1 and F2, respectively) are separated magnetically. Fractions B1 and B2 are, respectively, the bound cytoplasmic and cytoskeletal eluates and will contain candidate TbMORN1 binding partners and near neighbors. (B) Representative purification. Myc-BirA*-TbMORN1-expressing cells were processed as described for panel A. Samples were immunoblotted using HRP-conjugated streptavidin. Equal fractions were loaded in each lane. (C) Representative purification from Myc-BirA*-TbMORN1 cells incubated with excess biotin in either the presence or absence of tetracycline. Samples were immunoblotted using HRP-conjugated streptavidin; equal fractions in each lane. Comparison of +/− tetracycline conditions shows clear enrichment of biotinylated proteins in fractions E1, S1, and P1 and purification with minimal background in elutions B1 and B2 (left panel). Loading larger fractions of elutions B1 and B2 shows efficient capture of candidate TbMORN1 binding partners and near neighbors. Background signal from − Tet cells remains minimal (right panel).

Confirmation of expression of tagged candidate proteins. Whole-cell lysates from cells expressing candidate TbMORN1 binding partners and near neighbors were analyzed by immunoblotting with anti-Ty1 antibodies. (A) Western blot of candidates. Expected molecular size is shown next to the figure. Addition of the Ty1 epitope tag causes a distinct decrease in electrophoretic mobility. (B) Dot blot of the two larger candidates.

Localization of candidate proteins that overlap TbMORN1. Candidates were expressed with the Ty1 epitope tag in 427 cells either by transient transfection (first five rows) or endogenous replacement (last row). Detergent-extracted cells were labeled with anti-TbMORN1 and anti-Ty1 antibodies and analyzed by fluorescence microscopy. Single 0.1-μm z-slices are shown. Arrows indicate bilobe structure; area enlarged in insets. Fluorescence images were superimposed on differential interference contrast (DIC) images. The candidates localized either to the whole TbMORN1 bilobe (Tb927.10.3010, Tb927.8.3010) (A and B), primarily to the hook posterior part of TbMORN1 (Tb927.4.3120) (C), to both the bilobe and the adjacent TbCentrin4 arm (Tb927.7.7000, Tb927.10.850) (D and E), and to the posterior stem of TbMORN1 (Tb927.10.8820) (F). Scale bars, 2 μm.

Localization of candidate proteins to the bilobe, FAZ, and flagellum. Candidates were expressed with the Ty1 epitope tag in 427 cells either by endogenous replacement (first two rows) or transient transfection (last two rows). Detergent-extracted cells were labeled with anti-TbMORN1 and anti-Ty1 antibodies and analyzed by fluorescence microscopy. Tb927.10.1450 was localized adjacent to the anterior stem part of TbMORN1 (A). Tb927.7.3330 and Tb927.4.5340 were present on the FAZ and showed partial overlap with TbMORN1 (B and C). Tb927.7.3740 was present on the flagellum (D). Single 0.1-μm z-slices are shown. Arrows indicate bilobe structure; area enlarged in insets. Fluorescence images were superimposed on DIC images. Scale bars, 2 μm.

Localization of candidate TbMORN1 binding partners relative to TbCentrin4. Candidates were expressed with the Ty1 epitope tag in 427 cells either by transient transfection (first two rows) or endogenous replacement (last two rows). Detergent-extracted cells were labeled with anti-TbCentrin4 and anti-Ty1 antibodies and analyzed by fluorescence microscopy. Single 0.1-μm z-slices are shown. Arrows indicate bilobe structure; area enlarged in insets. Fluorescence images were superimposed on DIC images. As expected, Tb927.10.850 and Tb927.7.7000 showed a partial overlap with TbCentrin4 (A and B). Tb927.10.1450 (C) and Tb927.10.8820 (D) showed good and no overlap, respectively. Scale bars, 2 μm.