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Characterization of Three Ammonium Transporters of the Glomeromycotan Fungus Geosiphon pyriformis

Matthias Ellerbeck, Arthur Schüßler, David Brucker, Claudia Dafinger, Friedemann Loos, Andreas Brachmann
Matthias Ellerbeck
LMU Munich, Biocenter, Genetics, Martinsried, Germany
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Arthur Schüßler
LMU Munich, Biocenter, Genetics, Martinsried, Germany
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David Brucker
LMU Munich, Biocenter, Genetics, Martinsried, Germany
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Claudia Dafinger
LMU Munich, Biocenter, Genetics, Martinsried, Germany
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Friedemann Loos
LMU Munich, Biocenter, Genetics, Martinsried, Germany
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Andreas Brachmann
LMU Munich, Biocenter, Genetics, Martinsried, Germany
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DOI: 10.1128/EC.00139-13
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ABSTRACT

Members of the Glomeromycota form the arbuscular mycorrhiza (AM) symbiosis. They supply plants with inorganic nutrients, including nitrogen, from the soil. To gain insight into transporters potentially facilitating nitrogen transport processes, ammonium transporters (AMTs) of Geosiphon pyriformis, a glomeromycotan fungus forming a symbiosis with cyanobacteria, were studied. Three AMT genes were identified, and all three were expressed in the symbiotic stage. The localization and functional characterization of the proteins in a heterologous yeast system revealed distinct characteristics for each of them. AMT1 of G. pyriformis (GpAMT1) and GpAMT2 were both plasma membrane localized, but only GpAMT1 transported ammonium. Neither protein transported the ammonium analogue methylammonium. Unexpectedly, GpAMT3 was localized in the vacuolar membrane, and it has as-yet-unknown transport characteristics. An unusual cysteine residue in the AMT signature of GpAMT2 and GpAMT3 was identified, and the corresponding residue was demonstrated to play an important role in ammonium transport. Surprisingly, each of the three AMTs of G. pyriformis had very distinct features. The localization of an AMT in the yeast vacuolar membrane is novel, as is the described amino acid residue that clearly influences ammonium transport. The AMT characteristics might reflect adaptations to the lifestyle of glomeromycotan fungi.

FOOTNOTES

    • Received 11 June 2013.
    • Accepted 15 September 2013.
    • Accepted manuscript posted online 20 September 2013.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/EC.00139-13.

  • Copyright © 2013, American Society for Microbiology. All Rights Reserved.
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Characterization of Three Ammonium Transporters of the Glomeromycotan Fungus Geosiphon pyriformis
Matthias Ellerbeck, Arthur Schüßler, David Brucker, Claudia Dafinger, Friedemann Loos, Andreas Brachmann
Eukaryotic Cell Oct 2013, 12 (11) 1554-1562; DOI: 10.1128/EC.00139-13

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Characterization of Three Ammonium Transporters of the Glomeromycotan Fungus Geosiphon pyriformis
Matthias Ellerbeck, Arthur Schüßler, David Brucker, Claudia Dafinger, Friedemann Loos, Andreas Brachmann
Eukaryotic Cell Oct 2013, 12 (11) 1554-1562; DOI: 10.1128/EC.00139-13
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