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ARTICLE

Evolutionary Analyses of the Small Subunit of Glutamate Synthase: Gene Order Conservation, Gene Fusions, and Prokaryote-to- Eukaryote Lateral Gene Transfers

Jan O. Andersson, Andrew J. Roger
Jan O. Andersson
The Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
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  • For correspondence: joanders@is.dal.ca
Andrew J. Roger
The Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
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DOI: 10.1128/EC.1.2.304-310.2002
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  • FIG. 1.
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    FIG. 1.

    The ML tree of the inferred amino acid sequence of gltD, sudA, and gltD-like genes and their gene arrangements. See Materials and Methods for gene nomenclature. The different typefaces of the species names indicate whether the species belongs to the eukaryotes (all capitals), Archaea (lowercase, normal), or Bacteria (lowercase italic). Three main parts of the tree are indicated with large boxes, as follows: a clade with mostly gltD genes (A); a clade with only sudA genes (B); and a paraphyletic group with different gltD-like genes (C). The tree was arbitrarily rooted between the gltD clade and the paraphyletic group of gltD-like genes. Black boxes represent the homologous part of the gltD, gltD-like, and sudA sequences (used in analysis), open boxes represent gltB genes, gray boxes represent sudB genes, and boxes with dotted borders represent unique N- or C-terminal extensions of the gltD-like genes. Note that there is no detectable sequence homology between the extensions represented by dotted boxes. A thin line between two boxes indicates neighboring genes on the chromosome. Two boxes attached to each other indicate fusion of the genes. Protein ML-distance bootstrap values >50% for bipartitions are shown.

  • FIG. 2.
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    FIG. 2.

    Amino acid alignment of the iron-cluster binding motifs in sulfide dehydrogenase, proposed by Hagen et al. (15). The numbers above the alignments refer to the amino acid position in the P. furiosus SudB (cluster I) and SudA (cluster II and III) proteins. The proposed ligands are indicated by shadowed boxes. Note that cluster I has been proposed to have a novel Asp(Cys)3 binding motif (15).

  • FIG. 3.
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    FIG. 3.

    Phylogeny of concatenated sudA and sudB protein sequences. Protein ML bootstrap and ML distance bootstrap support values are shown above and below the branches, respectively. Only support values >50% for bipartitions are shown.

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Evolutionary Analyses of the Small Subunit of Glutamate Synthase: Gene Order Conservation, Gene Fusions, and Prokaryote-to- Eukaryote Lateral Gene Transfers
Jan O. Andersson, Andrew J. Roger
Eukaryotic Cell Apr 2002, 1 (2) 304-310; DOI: 10.1128/EC.1.2.304-310.2002

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Evolutionary Analyses of the Small Subunit of Glutamate Synthase: Gene Order Conservation, Gene Fusions, and Prokaryote-to- Eukaryote Lateral Gene Transfers
Jan O. Andersson, Andrew J. Roger
Eukaryotic Cell Apr 2002, 1 (2) 304-310; DOI: 10.1128/EC.1.2.304-310.2002
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KEYWORDS

Diplomonadida
Eukaryotic Cells
Evolution, Molecular
Gene Order
Gene Transfer, Horizontal
Glutamate Synthase
Prokaryotic Cells

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