Cover photograph (Copyright © 2005, American Society for Microbiology. All Rights Reserved.): Subcellular localization in budding yeast cells of the opportunistic fungal pathogen Candida albicans of the product of ABG1, a new and essential gene of this fungus, encoding a vacuolar protein (Abg1p). The ABG1 gene product was localized by its previous fusion with the green fluorescent protein (GFP) of Aequorea victoria. Fluorescence emitted by the fusion polypeptide (Abg1p-GFP) was exclusively localized on the membranous surface of vacuolar compartments. The cells were additionally stained with calcofluor white, a fluorescent dye (blue fluorescence) that specifically stains fungal cell walls. Cells were examined on wet mounts with a Leica DMR fluorescence microscope. (See related article on page 1088.)