Eukaryotic Cell doi:10.1128/EC.00377-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
Sexual development in C. neoformans requires CLP1, a target of the homeodomain transcription factors Sxi1
and Sxi2a
Joanne L. Ekena,
Brynne C. Stanton,
Jessica A. Schiebe-Owens,
and
Christina M. Hull*
Department of Biomolecular Chemistry; Department of Medical Microbiology & Immunology; University of Wisconsin, School of Medicine and Public Health, Madison, WI 53706
* To whom correspondence should be addressed. Email:
cmhull{at}wisc.edu.
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Abstract |
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Sexual development in the human fungal pathogen Cryptococcus neoformans is a multi-step process that results in the formation of spores, the likely infectious particles. A critical step in this developmental process is the transition from bud-form growth to filamentous growth. This transition is controlled by the homeodomain transcription factors Sxi1
and Sxi2a, the targets of which are largely unknown. Here we describe the discovery of a gene, CLP1, that is regulated by Sxi1 and Sxi2a and is essential for sexual development. In vitro binding studies also show that the CLP1 promoter is bound directly by Sxi1 and Sxi2a. The deletion of CLP1 leads to a block in sexual development after cell fusion but before filament formation, and cells without CLP1 are unable to grow vegetatively after cell fusion. Our findings lead to a model in which CLP1 is a downstream target of the Sxi proteins that functions to promote growth after mating and establish the filamentous state, a critical step in the production of spores.
