Eukaryotic Cell
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EC Accepts, published online ahead of print on 9 November 2007
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Eukaryotic Cell doi:10.1128/EC.00347-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

ChsVb, a class VII chitin synthase involved in septation, is critical for pathogenicity in Fusarium oxysporum

Magdalena Martín-Urdíroz, M. Isabel G. Roncero, José Antonio González-Reyes, and Carmen Ruiz-Roldán*

Departamento de Genética, Edif C5; Departamento de Biología Celular, Fisiología e Inmunología, Edif C6; Universidad de Córdoba, Campus Universitario de Rabanales, E-14071 Córdoba, Spain

* To whom correspondence should be addressed. Email: ge2rurom{at}uco.es.


  Abstract

A new myosin motor-like chitin synthase gene, chsVb, has been identified in the vascular wilt fungus Fusarium oxysporum f. sp. lycopersici. Phylogenetic analysis of the deduced amino acid sequence of chsVb chitin synthase 2 domain (CS2) revealed that ChsVb belongs to class VII chitin synthases. ChsVb myosin motor-like domain (MMD) is shorter than MMD of class V chitin synthases and does not contain typical ATP-binding motifs. Targeted disrupted {Delta}chsVb single and {Delta}chsV{Delta}chsVb double mutants were unable to infect and colonize tomato plants or to grow invasively on tomato fruit tissue. These strains were hypersensitive to compounds that interfere with fungal cell wall assembly, produced lemon-like shaped conidia and showed swollen balloon-like structures in hyphal subapical regions, thickened walls, aberrant septa and intrahyphal hyphae. Our results suggest that chsVb gene is likely to function in polarized growth and confirm the critical importance of cell wall integrity in the complex infection process of this fungus.






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