Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
EC Accepts, published online ahead of print on 2 February 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Other Versions of this Article:
EC.00340-06v1
6/4/682    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Argimón, S.
Right arrow Articles by Brown, A. J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Argimón, S.
Right arrow Articles by Brown, A. J. P.

 Previous Article  |  Next Article 

Eukaryotic Cell doi:10.1128/EC.00340-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Developmental regulation of an adhesin gene during cellular morphogenesis in the fungal pathogen Candida albicans

Silvia Argimón, Jill A. Wishart, Roger Leng, Susan Macaskill, Abigail Mavor, Thomas Alexandris, Susan Nicholls, Andrew W. Knight, Brice Enjalbert, Richard Walmsley, Frank C. Odds, Neil A.R. Gow, and Alistair J. P. Brown*

School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK; Gentronix Limited, CTF Building, 46 Grafton Street, Manchester, M13 9NT. UK

* To whom correspondence should be addressed. Email: al.brown{at}abdn.ac.uk.


  Abstract

Candida albicans expresses specific virulence traits that promote disease establishment and progression. These include morphological transitions between yeast and hyphal growth forms that are thought to contribute to dissemination and invasion, and cell surface adhesins that promote attachment to the host. Here we describe the regulation of the adhesin gene ALS3, which is expressed specifically during hyphal development in C. albicans. Using a combination of reporter constructs and regulatory mutants, we show that this regulation is mediated by multiple factors at the transcriptional level. The analysis of ALS3 promoter deletions revealed that this promoter contains two activation regions: one is essential for activation during hyphal development, whilst the second increases the amplitude of this activation. Further deletion analyses using the Renilla reniformis luciferase reporter delineate the essential activation region between -471 and -321 of the promoter. Further 5' or 3' deletions block activation. ALS3 transcription is repressed mainly by Nrg1 and Tup1, but Rfg1 contributes to this repression. Efg1, Tec1 and Bcr1 are essential for the transcriptional activation of ALS3, Tec1 mediating its effects indirectly through Bcr1 rather than through the putative Tec1 sites in the ALS3 promoter. ALS3 transcription is not affected by Cph2, but Cph1 contributes to full ALS3 activation. The data suggest that multiple morphogenetic signalling pathways operate through the promoter of this adhesin gene to mediate its developmental regulation in this major fungal pathogen.




This article has been cited by other articles:



HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2007 by the American Society for Microbiology.