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Eukaryotic Cell doi:10.1128/EC.00336-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Cryptococcus neoformans produces authentic PGE₂ without a cyclooxygenase

Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and Department of Microbiology and Immunology, University of Michigan Medical School

^* To whom correspondence should be addressed. Email: ghuff{at}umich.edu.

	Abstract

Many single-celled eukaryotes produce prostaglandin-like molecules, but these have not been absolutely verified by mass spectometry. We have isolated, and identified by LC-MS/MS, authentic PGE₂ from C. neoformans. Cyclooxygenase inhibitors did not inhibit prostaglandin synthesis and the cryptococcal genome lacks a cyclooxygenase homolog. Thus, novel enzymes must exist.