Eukaryotic Cell doi:10.1128/EC.00222-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
Endocytosis in the Shiitake Mushroom Lentinula edodes and the Involvement of GTPase Le.RAB7
Ming Tsung Lee,
Carol Ying Ying Szeto,
Tak Pan Ng,
and
Hoi Shan Kwan*
Department of Biology, the Chinese University of Hong Kong, Shatin, New Territories; Department of Biology and Molecular Biotechnology Program, the Chinese University of Hong Kong, Shatin, New Territories
* To whom correspondence should be addressed. Email:
hoishankwan{at}cuhk.edu.hk.
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Abstract |
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Endocytosis is the process by which substrates enter a cell without passing through the plasma membrane but rather invaginate the cell membrane and form intracellular vesicles. Rab7 regulates endocytic trafficking between early and late endosomes and between late endosomes and lysosomes. Le.Rab7 in Lentinula edodes is strongly homologous to Rab7 in Homo sapiens. Receptors for activated C kinase-1 (Le.RACK1) and Rab5 GTPase (Le.RAB5) were isolated as interacting partners of Rab7, and the interactions were confirmed by in vivo and in vitro protein interaction assays. The three genes showed differential expression in the various developmental stages of the mushroom. In situ hybridization showed that the three transcripts were localized in regions of active growth, such as the outer region of trama cells, and the subhymenium of the hymenophore of mature fruiting bodies and the prehymenophore of young fruiting bodies. The existence of endocytosis in the mycelium and hymenophores was confirmed by the internalization of FM4-64. Le.RAB7 was partially colocalized with the AM4-64, and was located in the late endocytic pathway. This is the first report of the presence of endocytosis in homobasidiomycetes. Le.RAB7, Le.RAB5 and Le.RACK1 may contribute to the growth of L. edodes and cell differentiation in hymenophores.
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