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Eukaryotic Cell doi:10.1128/EC.00184-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Nucleosome positioning and histone H3-acetylation are independent processes in the Aspergillus nidulans prnD-prnB bidirectional promoter

Fungal Genomics Unit, Austrian Research Centers and BOKU Vienna, A-1190 Vienna, Austria, Istitute de Genetique et Microbiologie, Université Paris-Sud, F-91495 Orsay-CEDEX, France

^* To whom correspondence should be addressed. Email: joseph.strauss{at}boku.ac.at.

	Abstract

In Aspergillus nidulans, proline can be used as carbon and nitrogen source and its metabolism requires the integration of three signals including proline induction, and nitrogen and carbon metabolite de-repression. We have previously shown that the bidirectional promoter in the prnD-prnB intergenic region undergoes drastic chromatin rearrangements where proline induction leads to loss of positioned nucleosomes whereas simultaneous carbon and nitrogen metabolite repression results in partial re-positioning of these nucleosomes. In the proline cluster, inhibition of deacetylases by trichostatin A (TSA), leads to partial de-repression and is associated with a lack of nucleosome positioning. Here, we investigate the effect of histone acetylation in the proline cluster using strains deleted for essential components of putative A. nidulans histone acetyl transferase (HAT) complexes, namely gcnE and adaB, the orthologues of the S. cerevisiae GCN5 and ADA2 genes, respectively. Surprisingly, GcnE and AdaB are not required for transcriptional activation and chromatin remodelling but for repression of prnB and prnD and for re-positioning of nucleosomes in the divergent promoter region. Chromatin immunoprecipitation (ChIP) directed against histone H3 lysines K9 and K14 revealed that GcnE and AdaB participate in increasing the acetylation level of at least one nucleosome in the prnD-prnB intergenic region during activation, but these activities do not determine nucleosome positioning. Our results are consistent with a function of GcnE and AdaB in gene repression of the proline cluster, probably an indirect effect related to the function of CreA, the DNA-binding protein mediating carbon catabolite repression in Aspergillus nidulans.

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