Eukaryotic Cell
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EC Accepts, published online ahead of print on 9 November 2007
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EC.00055-07v1
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Eukaryotic Cell doi:10.1128/EC.00055-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A new assay for promoter analysis in Chlamydomonas reveals a role for heat shock elements and TATA-box in HSP70A promoter mediated activation of transgene expression

Mukesh Lodha, Miriam Schulz-Raffelt, and Michael Schroda*

Institute of Biology II, Plant Biochemistry, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany

* To whom correspondence should be addressed. Email: michael.schroda{at}biologie.uni-freiburg.de.


  Abstract

The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat-shock elements and the TATA-box.


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