EC Accepts, published online ahead of print on 17 April 2009

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Eukaryotic Cell doi:10.1128/EC.00041-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Using morpholinos for gene knockdown in Giardia intestinalis

Meredith L. Carpenter and W. Zacheus Cande^*

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720

^* To whom correspondence should be addressed. Email: zcande{at}berkeley.edu.

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either GFP or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter of morpholino efficacy for targets lacking specific antibodies.