Eukaryotic Cell
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EC Accepts, published online ahead of print on 23 February 2007
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Eukaryotic Cell doi:10.1128/EC.00020-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The MAT1 locus of Histoplasma capsulatum is responsive in a mating type specific manner

Meggan Bubnick and A. George Smulian*

Department of Pathology, Infectious Disease Division, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0560, and Cincinnati VA Medical Center, 3200 Vine Street, Cincinnati, OH 45220

* To whom correspondence should be addressed. Email: alan.smulian{at}uc.edu.


  Abstract

Recombination events associated with sexual replication in pathogens may generate new strains with altered virulence. Histoplasma capsulatum is a mating-competent, pathogenic fungus with two described phenotypic mating types, (+) and (-). The mating (MAT) locus of H. capsulatum was identified to facilitate molecular studies of mating in this organism. Through syntenic analysis of the H. capsulatum genomic sequence databases, a MAT1-1 idiomorph region was identified in H. capsulatum strains G217B and WU24, and a MAT1-2 idiomorph region was identified in the strain G186AR. A mating-type specific PCR assay was developed and two clinical isolates of opposite genotypic mating type, UH1 and VA1, were identified. A known (-) mating type strain, T-3-1 (ATCC 22635), was demonstrated to be of MAT1-2 genotypic mating type. The clinical isolates UH1 and VA1 were found to be mating compatible, and also displayed mating-type dependent regulation of the MAT transcription factors in response to extracts predicted to contain mating pheromone. These studies support a role for the identified MAT1 locus in determining mating type in H. capsulatum.




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