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Eukaryotic Cell, June 2009, p. 913-915, Vol. 8, No. 6
1535-9778/09/$08.00+0     doi:10.1128/EC.00394-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Flavin Mononucleotide-Based Fluorescent Protein as an Oxygen-Independent Reporter in Candida albicans and Saccharomyces cerevisiae{triangledown} ,{dagger}

D. Tielker,1 I. Eichhof,1 K.-E. Jaeger,2 and J. F. Ernst1*

Institut für Mikrobiologie,1 Institut für Molekulare Enzymtechnologie, Forschungszentrum Jülich, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany2

Received 19 December 2008/ Accepted 9 April 2009

Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (CaFbFP) was expressed in Candida albicans and Saccharomyces cerevisiae. Both species produced CaFbFP and fluoresced even during hypoxia, suggesting that oxygen-independent CaFbFP is a useful, novel tool for monitoring hypoxic gene expression in fungi.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Molekulare Mykologie, Universitätsstr. 1/26.12, 40225 Düsseldorf, Germany. Phone and fax: 49(211)811-5176. E-mail: joachim.ernst{at}uni-duesseldorf.de

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, June 2009, p. 913-915, Vol. 8, No. 6
1535-9778/09/$08.00+0     doi:10.1128/EC.00394-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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