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Eukaryotic Cell, September 2008, p. 1450-1459, Vol. 7, No. 9
1535-9778/08/$08.00+0     doi:10.1128/EC.00168-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Regulation of the Subcellular Localization of Cyclic AMP-Dependent Protein Kinase in Response to Physiological Stresses and Sexual Differentiation in the Fission Yeast Schizosaccharomyces pombe{triangledown} ,{dagger}

Yasuhiro Matsuo, Brittney McInnis, and Stevan Marcus*

Department of Biological Sciences, The University of Alabama, Tuscaloosa, Alabama 35487

Received 13 May 2008/ Accepted 26 June 2008

We describe regulation of the subcellular localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) regulatory (Cgs1p) and catalytic (Pka1p) subunits in the fission yeast Schizosaccharomyces pombe in response to physiological stresses and during sexual differentiation as determined by fluorescence microscopy of the Cgs1-green fluorescent protein (GFP) and Pka1-GFP fusion proteins, respectively. In wild-type S. pombe cells cultured to log phase under normal growth conditions, Cgs1p and Pka1p are concentrated in the nucleus and more diffusely present in the cytoplasm. Nuclear localization of both proteins is dependent on cAMP, since in cells lacking adenylate cyclase they are detectable only in the cytoplasm. In cells lacking Cgs1p or both Cgs1p and adenylate cyclase, Pka1p is concentrated in the nucleus, demonstrating a role for Cgs1p in the nuclear exclusion of Pka1p. Nuclear-cytoplasmic redistribution of Cgs1p and Pka1p is triggered by growth in glucose-limited or hyperosmotic media and in response to stationary-phase growth. In addition, both proteins are excluded from the nucleus in mating cells undergoing karyogamy and subsequently concentrated in postmeiotic spores. Cgs1p is required for subcellular redistribution of Pka1p induced by growth in glucose-limited and hyperosmotic media and during karyogamy but is not required for Pka1p redistribution triggered by stationary-phase growth or for the enrichment of Pka1p in spores. Our results demonstrate that PKA localization is regulated by cAMP and regulatory subunit-dependent and -independent mechanisms in S. pombe.

* Corresponding author. Mailing address: Department of Biological Sciences, The University of Alabama, 343A Shelby Hall, 250 Hackberry Lane, Tuscaloosa, AL 35487. Phone: (205) 348-8094. Fax: (205) 348-9104. E-mail: smarcus{at}bama.ua.edu

{triangledown} Published ahead of print on 11 July 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, September 2008, p. 1450-1459, Vol. 7, No. 9
1535-9778/08/$08.00+0     doi:10.1128/EC.00168-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roux, A. E., Chartrand, P., Ferbeyre, G., Rokeach, L. A. (2009). Fission Yeast and Other Yeasts as Emergent Models to Unravel Cellular Aging in Eukaryotes. J Gerontol A Biol Sci Med Sci 0: glp152v1-glp152 [Abstract] [Full Text]  


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