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Eukaryotic Cell, April 2008, p. 630-638, Vol. 7, No. 4
1535-9778/08/$08.00+0     doi:10.1128/EC.00425-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Aspergillus calidoustus sp. nov., Causative Agent of Human Infections Previously Assigned to Aspergillus ustus{triangledown}

János Varga,1,2 Jos Houbraken,1 Henrich A. L. Van Der Lee,3 Paul E. Verweij,3 and Robert A. Samson1*

CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands,1 Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, P.O. Box 533, Hungary,2 Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen University Center for Infectious Diseases, P.O. Box 9101 6500 HB, Nijmegen, The Netherlands3

Received 20 November 2007/ Accepted 7 February 2008

Aspergillus ustus is a relatively rare human pathogen causing invasive infections in immunocompromised hosts. In this study isolates originating from clinical and other sources have been examined using molecular, morphological, and physiological approaches to clarify their species assignment. Phylogenetic analysis of partial β-tubulin, calmodulin, actin, and intergenic transcribed spacer sequences indicated that none of the clinical isolates recognized previously as A. ustus belongs to this species. All but two of these isolates formed a well-defined clade related to A. pseudodeflectus based on sequence analysis of protein-coding regions. Morphological and physiological examination of these isolates indicated that they are able to grow above 37°C, in contrast with A. ustus isolates, and give a positive Ehrlich reaction, in contrast with related species including A. granulosus, A. ustus, and A. pseudodeflectus. These isolates are proposed as a new species, A. calidoustus. Antifungal susceptibility testing showed that this species has decreased susceptibilities to several antifungal drugs. The triazoles are inactive in vitro, including the new azole posaconazole.

* Corresponding author. Mailing address: CBS Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. Phone: 31 30 2122600. Fax: 31 30 2512097. E-mail: r.samson{at}cbs.knaw.nl

{triangledown} Published ahead of print on 15 February 2008.

Eukaryotic Cell, April 2008, p. 630-638, Vol. 7, No. 4
1535-9778/08/$08.00+0     doi:10.1128/EC.00425-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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