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 Previous Article

Eukaryotic Cell, November 2008, p. 2012-2016, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00198-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

African Trypanosomes Contain 5-Methylcytosine in Nuclear DNA {triangledown} ,{dagger}

Kevin T. Militello,1* Ping Wang,2 Sangeeta K. Jayakar,1 Rebecca L. Pietrasik,1 Christopher D. Dupont,1,{ddagger} Kristi Dodd,1 Anthony M. King,1 and Paul R. Valenti1,§

State University of New York at Geneseo, Department of Biology, Geneseo, New York,1 Roswell Park Cancer Institute, Pharmacokinetics/Pharmacodynamics Facility, Buffalo, New York2

Received 17 June 2008/ Accepted 2 September 2008

It is currently unclear if there are modified DNA bases in Trypanosoma brucei other than J-base. We identify herein a cytosine-5 DNA methyltransferase gene and report the presence and location of 5-methylcytosine in genomic DNA. Our data demonstrate that African trypanosomes contain a functional cytosine DNA methylation pathway.

* Corresponding author. Mailing address: State University of New York at Geneseo, Integrated Science Center 341, 1 College Circle, Geneseo, NY 14454. Phone: (585) 245-5312. Fax: (585) 245-5007. E-mail: militello{at}geneseo.edu

{triangledown} Published ahead of print on 12 September 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: University of Pennsylvania, Department of Pathobiology, Philadelphia, PA.

§ Present address: University of Rochester Medical Center, Infectious Disease Unit, Rochester, NY.

Eukaryotic Cell, November 2008, p. 2012-2016, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00198-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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