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Eukaryotic Cell, November 2008, p. 1941-1950, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00249-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

KMP-11, a Basal Body and Flagellar Protein, Is Required for Cell Division in Trypanosoma brucei{triangledown}

Ziyin Li and Ching C. Wang*

Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94158-2280

Received 23 July 2008/ Accepted 14 September 2008

Kinetoplastid membrane protein 11 (KMP-11) has been identified as a flagellar protein and is conserved among kinetoplastid parasites, but its potential function remains unknown. In a recent study, we identified KMP-11 as a microtubule-bound protein localizing to the flagellum as well as the basal body in both procyclic and bloodstream forms of Trypanosoma brucei (Z. Li, J. H. Lee, F. Chu, A. L. Burlingame, A. Gunzl, and C. C. Wang, PLoS One 3:e2354, 2008). Silencing of KMP-11 by RNA interference inhibited basal body segregation and cytokinesis in both forms and resulted in multiple nuclei of various sizes, indicating a continuous, albeit somewhat defective, nuclear division while cell division was blocked. KMP-11 knockdown in the procyclic form led to severely compromised formation of the new flagellum attachment zone (FAZ) and detachment of the newly synthesized flagellum. However, a similar phenotype was not observed in the bloodstream form depleted of KMP-11. Thus, KMP-11 is a flagellar protein playing critical roles in regulating cytokinesis in both forms of the trypanosomes. Its distinct roles in regulating FAZ formation in the two forms may provide a clue to the different mechanisms of cytokinetic initiation in procyclic and bloodstream trypanosomes.

* Corresponding author. Mailing address: Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158-2280. Phone: (415) 476-1321. Fax: (415) 476-3382. E-mail: ccwang{at}cgl.ucsf.edu

{triangledown} Published ahead of print on 26 September 2008.

Eukaryotic Cell, November 2008, p. 1941-1950, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00249-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Portman, N., Lacomble, S., Thomas, B., McKean, P. G., Gull, K. (2009). Combining RNA Interference Mutants and Comparative Proteomics to Identify Protein Components and Dependences in a Eukaryotic Flagellum. J. Biol. Chem. 284: 5610-5619 [Abstract] [Full Text]  


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