Eukaryotic Cell
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Eukaryotic Cell, January 2008, p. 78-85, Vol. 7, No. 1
1535-9778/08/$08.00+0     doi:10.1128/EC.00362-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Protein Kinases Involved in Mating and Osmotic Stress in the Yeast Kluyveromyces lactis{triangledown}

Laura Kawasaki,1 María Castañeda-Bueno,1 Edith Sánchez-Paredes,1 Nancy Velázquez-Zavala,1 Francisco Torres-Quiroz,1 Laura Ongay-Larios,2 and Roberto Coria1*

Departamento de Genética Molecular,1 Unidad de Biología Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510 México D. F., Mexico2

Received 2 October 2007/ Accepted 29 October 2007

Systematic disruption of genes encoding kinases and mitogen-activated protein kinases (MAPKs) was performed in Kluyveromyces lactis haploid cells. The mutated strains were assayed by their capacity to mate and to respond to hyperosmotic stress. The K. lactis Ste11p (KlSte11p) MAPK kinase kinase (MAPKKK) was found to act in both mating and osmoresponse pathways while the scaffold KlSte5p and the MAPK KlFus3p appeared to be specific for mating. The p21-activated kinase KlSte20p and the kinase KlSte50p participated in both pathways. Protein association experiments showed interaction of KlSte50p and KlSte20p with G{alpha} and Gβ, respectively, the G protein subunits involved in the mating pathway. Both KlSte50p and KlSte20p also showed interaction with KlSte11p. Disruption mutants of the K. lactis PBS2 (KlPBS2) and KlHOG1 genes of the canonical osmotic response pathway resulted in mutations sensitive to high salt and high sorbitol but dispensable for mating. Mutations that eliminate the MAPKK KlSte7p activity had a strong effect on mating and also showed sensitivity to osmotic stress. Finally, we found evidence of physical interaction between KlSte7p and KlHog1p, in addition to diminished Hog1p phosphorylation after a hyperosmotic shock in cells lacking KlSte7p. This study reveals novel roles for components of transduction systems in yeast.

* Corresponding author. Mailing address: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510 México, D. F. México. Phone: (525) 622 56 52. Fax: (525) 622 56 30. E-mail: rcoria{at}ifc.unam.mx

{triangledown} Published ahead of print on 16 November 2007.

Eukaryotic Cell, January 2008, p. 78-85, Vol. 7, No. 1
1535-9778/08/$08.00+0     doi:10.1128/EC.00362-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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