This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, F.
Right arrow Articles by Palecek, S. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, F.
Right arrow Articles by Palecek, S. P.

 Previous Article  |  Next Article 

Eukaryotic Cell, June 2007, p. 931-939, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00049-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation In Vitro and In Vivo{triangledown}

Fang Li,1,{dagger} Michael J. Svarovsky,1,{ddagger} Amy J. Karlsson,1 Joel P. Wagner,1 Karen Marchillo,2 Philip Oshel,3 David Andes,2 and Sean P. Palecek1*

Department of Chemical & Biological Engineering,1 Department of Medicine,2 Department of Animal Sciences, University of Wisconsin—Madison, Madison, Wisconsin 537063

Received 19 February 2007/ Accepted 26 March 2007

Candida albicans is the leading cause of systemic fungal infections in immunocompromised humans. The ability to form biofilms on surfaces in the host or on implanted medical devices enhances C. albicans virulence, leading to antimicrobial resistance and providing a reservoir for infection. Biofilm formation is a complex multicellular process consisting of cell adhesion, cell growth, morphogenic switching between yeast form and filamentous states, and quorum sensing. Here we describe the role of the C. albicans EAP1 gene, which encodes a glycosylphosphatidylinositol-anchored, glucan-cross-linked cell wall protein, in adhesion and biofilm formation in vitro and in vivo. Deleting EAP1 reduced cell adhesion to polystyrene and epithelial cells in a gene dosage-dependent manner. Furthermore, EAP1 expression was required for C. albicans biofilm formation in an in vitro parallel plate flow chamber model and in an in vivo rat central venous catheter model. EAP1 expression was upregulated in biofilm-associated cells in vitro and in vivo. Our results illustrate an association between Eap1p-mediated adhesion and biofilm formation in vitro and in vivo.

* Corresponding author. Mailing address: Department of Chemical & Biological Engineering, 1415 Engineering Drive, Madison, WI 53706. Phone: (608) 262-8931. Fax: (608) 262-5434. E-mail: palecek{at}engr.wisc.edu

{triangledown} Published ahead of print on 6 April 2007.

{dagger} Present address: Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 200 1st St. SW, Rochester, MN 55905.

{ddagger} Present address: 3M Corporate Research Materials Laboratory, 201-3E-03, St. Paul, MN 55144.

Eukaryotic Cell, June 2007, p. 931-939, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00049-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • MacCallum, D. M., Castillo, L., Nather, K., Munro, C. A., Brown, A. J. P., Gow, N. A. R., Odds, F. C. (2009). Property Differences among the Four Major Candida albicans Strain Clades. Eukaryot Cell 8: 373-387 [Abstract] [Full Text]  
  • ten Cate, J.M., Klis, F.M., Pereira-Cenci, T., Crielaard, W., de Groot, P.W.J. (2009). Molecular and Cellular Mechanisms That Lead to Candida Biofilm Formation. JDR 88: 105-115 [Abstract] [Full Text]  
  • Chaffin, W. L. (2008). Candida albicans Cell Wall Proteins. Microbiol. Mol. Biol. Rev. 72: 495-544 [Abstract] [Full Text]  
  • Li, F., Palecek, S. P. (2008). Distinct domains of the Candida albicans adhesin Eap1p mediate cell-cell and cell-substrate interactions. Microbiology 154: 1193-1203 [Abstract] [Full Text]  
  • Sosinska, G. J., de Groot, P. W. J., Teixeira de Mattos, M. J., Dekker, H. L., de Koster, C. G., Hellingwerf, K. J., Klis, F. M. (2008). Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions. Microbiology 154: 510-520 [Abstract] [Full Text]  
  • Norice, C. T., Smith, F. J. Jr., Solis, N., Filler, S. G., Mitchell, A. P. (2007). Requirement for Candida albicans Sun41 in Biofilm Formation and Virulence. Eukaryot Cell 6: 2046-2055 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»