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 Previous Article

Eukaryotic Cell, June 2007, p. 1063-1067, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00072-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Chlamydomonas reinhardtii CNX1E Reconstitutes Molybdenum Cofactor Biosynthesis in Escherichia coli Mutants{triangledown} ,{dagger}

Ángel Llamas,1 Manuel Tejada-Jimenez,1 David González-Ballester,1,{ddagger} José Javier Higuera,1 Guenter Schwarz,2 Aurora Galván,1 and Emilio Fernández1*

Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, Córdoba 14071, Spain,1 Institut für Biochemie, Universität zu Köln, Otto-Fischer-Str. 12-14, 50674 Köln, Germany2

Received 9 March 2007/ Accepted 9 March 2007

We have isolated and characterized the Chlamydomonas reinhardtii genes for molybdenum cofactor biosynthesis, namely, CNX1G and CNX1E, and expressed them and their chimeric fusions in Chlamydomonas and Escherichia coli. In all cases, the wild-type phenotype was restored in individual mutants as well as in a CNX1G CNX1E double mutant. Therefore, CrCNX1E is the first eukaryotic protein able to complement an E. coli moeA mutant.

* Corresponding author. Mailing address: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edif. Severo Ochoa, 14071 Córdoba, Spain. Phone and fax: 34-957-218591. E-mail: bb1feree{at}uco.es

{triangledown} Published ahead of print on 6 April 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: Department of Plant Biology, The Carnegie Institution of Washington, Stanford University, 260 Panama Street, Stanford, CA 94305.

Eukaryotic Cell, June 2007, p. 1063-1067, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00072-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fernandez, E., Galvan, A. (2008). Nitrate Assimilation in Chlamydomonas. Eukaryot Cell 7: 555-559 [Full Text]  


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