This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Westfall, P. J.
Right arrow Articles by Thorner, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Westfall, P. J.
Right arrow Articles by Thorner, J.

 Previous Article  |  Next Article 

Eukaryotic Cell, August 2006, p. 1215-1228, Vol. 5, No. 8
1535-9778/06/$08.00+0     doi:10.1128/EC.00037-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analysis of Mitogen-Activated Protein Kinase Signaling Specificity in Response to Hyperosmotic Stress: Use of an Analog-Sensitive HOG1 Allele

Patrick J. Westfall and Jeremy Thorner*

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720

Received 6 February 2006/ Accepted 23 May 2006

When confronted with a marked increase in external osmolarity, budding yeast (Saccharomyces cerevisiae) cells utilize a conserved mitogen-activated protein kinase (MAPK) signaling cascade (the high-osmolarity glycerol or HOG pathway) to elicit cellular responses necessary to permit continued growth. One input that stimulates the HOG pathway requires the integral membrane protein and putative osmosensor Sho1, which recruits and enables activation of the MAPK kinase kinase Ste11. In mutants that lack the downstream MAPK kinase (pbs2{Delta}) or the MAPK (hog1{Delta}) of the HOG pathway, Ste11 activated by hyperosmotic stress is able to inappropriately stimulate the pheromone response pathway. This loss of signaling specificity is known as cross talk. To determine whether it is the Hog1 polypeptide per se or its kinase activity that is necessary to prevent cross talk, we constructed a fully functional analog-sensitive allele of HOG1 to permit acute inhibition of this enzyme without other detectable perturbations of the cell. We found that the catalytic activity of Hog1 is required continuously to prevent cross talk between the HOG pathway and both the pheromone response and invasive growth pathways. Moreover, contrary to previous reports, we found that the kinase activity of Hog1 is necessary for its stress-induced nuclear import. Finally, our results demonstrate a role for active Hog1 in maintaining signaling specificity under conditions of persistently high external osmolarity.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Room 16, Barker Hall, University of California, Berkeley, Berkeley, CA 94720-3202. Phone: (510) 642-2558. Fax: (510) 642-6420. E-mail: jthorner{at}berkeley.edu.

Eukaryotic Cell, August 2006, p. 1215-1228, Vol. 5, No. 8
1535-9778/06/$08.00+0     doi:10.1128/EC.00037-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Birkaya, B., Maddi, A., Joshi, J., Free, S. J., Cullen, P. J. (2009). Role of the Cell Wall Integrity and Filamentous Growth Mitogen-Activated Protein Kinase Pathways in Cell Wall Remodeling during Filamentous Growth. Eukaryot Cell 8: 1118-1133 [Abstract] [Full Text]  
  • Pitoniak, A., Birkaya, B., Dionne, H. M., Vadaie, N., Cullen, P. J. (2009). The Signaling Mucins Msb2 and Hkr1 Differentially Regulate the Filamentation Mitogen-activated Protein Kinase Pathway and Contribute to a Multimodal Response. Mol. Biol. Cell 20: 3101-3114 [Abstract] [Full Text]  
  • Shock, T. R., Thompson, J., Yates, J. R. III, Madhani, H. D. (2009). Hog1 Mitogen-Activated Protein Kinase (MAPK) Interrupts Signal Transduction between the Kss1 MAPK and the Tec1 Transcription Factor To Maintain Pathway Specificity. Eukaryot Cell 8: 606-616 [Abstract] [Full Text]  
  • Macia, J., Regot, S., Peeters, T., Conde, N., Sole, R., Posas, F. (2009). Dynamic Signaling in the Hog1 MAPK Pathway Relies on High Basal Signal Transduction. Sci Signal 2: ra13-ra13 [Abstract] [Full Text]  
  • Westfall, P. J., Patterson, J. C., Chen, R. E., Thorner, J. (2008). Stress resistance and signal fidelity independent of nuclear MAPK function. Proc. Natl. Acad. Sci. USA 105: 12212-12217 [Abstract] [Full Text]  
  • Migdal, I., Ilina, Y., Tamas, M. J., Wysocki, R. (2008). Mitogen-Activated Protein Kinase Hog1 Mediates Adaptation to G1 Checkpoint Arrest during Arsenite and Hyperosmotic Stress. Eukaryot Cell 7: 1309-1317 [Abstract] [Full Text]  
  • Wang, C., Duan, Z., St. Leger, R. J. (2008). MOS1 Osmosensor of Metarhizium anisopliae Is Required for Adaptation to Insect Host Hemolymph. Eukaryot Cell 7: 302-309 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»