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Eukaryotic Cell, July 2006, p. 1026-1035, Vol. 5, No. 7
1535-9778/06/$08.00+0     doi:10.1128/EC.00129-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Changing Roles of Aurora-B Kinase in Two Life Cycle Stages of Trypanosoma brucei{dagger}

Ziyin Li and C. C. Wang*

Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94158-2280

Received 1 May 2006/ Accepted 3 May 2006

Aurora-B kinase is a chromosomal passenger protein essential for chromosome segregation and cytokinesis. In the procyclic form of Trypanosoma brucei, depletion of an aurora-B kinase homologue TbAUK1 inhibited spindle formation, mitosis, cytokinesis, and organelle replication without altering cell morphology. In the present study, an RNA interference knockdown of TbAUK1 or overexpression of inactive mutant TbAUK1-K58R in the bloodstream form also resulted in defects in spindle formation, chromosome segregation, and cytokinesis but allowed multiple rounds of nuclear DNA synthesis, nucleolus multiplication, and continuous replication of kinetoplast, basal body, and flagellum. The typical trypanosome morphology was lost to an enlarged round shape filled with microtubules. It is thus apparent that there are distinctive mechanisms of action of TbAUK1 in regulating cell division between the two developmental stages of trypanosome. While it exerts a tight control on mitosis, organelle replication, and cytokinesis in the procyclic form, it regulates cytokinesis without rigid control over either nuclear DNA synthesis or organelle replication in the bloodstream form. The molecular basis underlining these discrepancies remains to be explored.

* Corresponding author. Mailing address: Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158-2280. Phone: (415) 476-1321. Fax: (415) 476-3382. E-mail: ccwang{at}cgl.ucsf.edu.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, July 2006, p. 1026-1035, Vol. 5, No. 7
1535-9778/06/$08.00+0     doi:10.1128/EC.00129-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, Z., Wang, C. C. (2008). KMP-11, a Basal Body and Flagellar Protein, Is Required for Cell Division in Trypanosoma brucei. Eukaryot Cell 7: 1941-1950 [Abstract] [Full Text]  
  • Umeyama, T., Wang, C. C. (2008). Polo-Like Kinase Is Expressed in S/G2/M Phase and Associated with the Flagellum Attachment Zone in both Procyclic and Bloodstream Forms of Trypanosoma brucei. Eukaryot Cell 7: 1582-1590 [Abstract] [Full Text]  
  • Li, Z., Gourguechon, S., Wang, C. C. (2007). Tousled-like kinase in a microbial eukaryote regulates spindle assembly and S-phase progression by interacting with Aurora kinase and chromatin assembly factors. J. Cell Sci. 120: 3883-3894 [Abstract] [Full Text]  
  • Griffiths, S., Portman, N., Taylor, P. R., Gordon, S., Ginger, M. L., Gull, K. (2007). RNA Interference Mutant Induction In Vivo Demonstrates the Essential Nature of Trypanosome Flagellar Function during Mammalian Infection. Eukaryot Cell 6: 1248-1250 [Abstract] [Full Text]  
  • Boucher, N., Dacheux, D., Giroud, C., Baltz, T. (2007). An Essential Cell Cycle-regulated Nucleolar Protein Relocates to the Mitotic Spindle Where It Is Involved in Mitotic Progression in Trypanosoma brucei. J. Biol. Chem. 282: 13780-13790 [Abstract] [Full Text]  


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