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Eukaryotic Cell, April 2005, p. 716-721, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.716-721.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Role of Snf1p in Regulation of Intracellular Sorting of the Lactose and Galactose Transporter Lac12p in Kluyveromyces lactis{dagger}

Christian Wiedemuth and Karin D. Breunig*

Institut für Genetik, Martin-Luther-Universität Halle-Wittenberg, Halle, Germany

Received 30 July 2004/ Accepted 4 February 2005

The protein kinase Snf1/AMPK plays a central role in carbon and energy homeostasis in yeasts and higher eukaryotes. To work out which aspects of the Snf1-controlled regulatory network are conserved in evolution, the Snf1 requirement in galactose metabolism was analyzed in the yeast Kluyveromyces lactis. Whereas galactose induction was only delayed, K. lactis snf1 mutants failed to accumulate the lactose/galactose H+ symporter Lac12p in the plasma membran,e as indicated by Lac12-green fluorescent protein fusions. In contrast to wild-type cells, the fusion protein was mostly intracellular in the mutant. Growth on galactose and galactose uptake could be restored by the KHT3 gene, which encodes a new transporter of the HXT subfamily of major facilitators These findings indicate a new role of Snf1p in regulation of sugar transport in K. lactis.

* Corresponding author. Mailing address: Institut für Genetik, Martin-Luther-Universität Halle-Wittenberg, 06099 Halle, Germany. Phone: 49 345 5526304. Fax: 49 345 5527151. E-mail: karin.breunig{at}genetik.uni-halle.de.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, April 2005, p. 716-721, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.716-721.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Suleau, A., Gourdon, P., Reitz-Ausseur, J., Casaregola, S. (2006). Transcriptomic Analysis of Extensive Changes in Metabolic Regulation in Kluyveromyces lactis Strains.. Eukaryot Cell 5: 1360-1370 [Abstract] [Full Text]  


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