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Eukaryotic Cell, February 2005, p. 490-492, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.490-492.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Silenced Plasmodium falciparum var Promoter Can Be Activated In Vivo through Spontaneous Deletion of a Silencing Element in the Intron{dagger}

Laïla Gannoun-Zaki,1 Amy Jost,1 Jianbing Mu,1 Kirk W. Deitsch,2 and Thomas E. Wellems1*

Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland,1 Department of Microbiology and Immunology, Weill Medical College, Cornell University, New York, New York2

Received 7 September 2004/ Accepted 17 November 2004

Introns of Plasmodium falciparum var genes act as transcriptional silencing elements that help control antigenic variations. In transfected episomes, intron silencing of a drug-selectable marker under var promoter control is reversed by the spontaneous deletion of key intron regions. The resulting promoter activation does not affect the transcription of chromosomal var genes.

* Corresponding author. Mailing address: LMVR, NIAID, 12735 Twinbrook Pkwy., Room 3E-10D, Bethesda, MD 20892-8132. Phone: (301) 496-4021. Fax: (301) 402-2201. E-mail: tew{at}helix.nih.gov.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, February 2005, p. 490-492, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.490-492.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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