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Eukaryotic Cell, February 2005, p. 487-489, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.487-489.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Thioredoxin Reductase Is Essential for Viability in the Fungal Pathogen Cryptococcus neoformans

Tricia A. Missall1 and Jennifer K. Lodge1,2*

Edward A. Doisy Department of Biochemistry and Molecular Biology,1 Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, Missouri2

Received 4 November 2004/ Accepted 17 November 2004

Thioredoxin reductase (TRR1) is an important component of the thioredoxin oxidative stress resistance pathway. Here we show that it is induced during oxidative and nitrosative stress and is preferentially localized to the mitochondria in Cryptococcus neoformans. The C. neoformans TRR1 gene encodes the low-molecular-weight isoform of the thioredoxin reductase enzyme, which shares little homology with that of its mammalian host. By replacing the endogenous TRR1 promoter with an inducible copper transporter promoter, we showed that Trr1 appears to be essential for viability of this pathogenic fungus, making it a potential antifungal target.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 S. Grand Blvd., St. Louis, MO 63104. Phone: (314) 977-9217. Fax: (314) 977-9205. E-mail: lodgejk{at}slu.edu.

Eukaryotic Cell, February 2005, p. 487-489, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.487-489.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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