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Eukaryotic Cell, February 2005, p. 401-406, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.401-406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Chromosomal Translocation and Segmental Duplication in Cryptococcus neoformans{dagger}

James A. Fraser,1,2 Johnny C. Huang,1 Read Pukkila-Worley,1,3 J. Andrew Alspaugh,1,3 Thomas G. Mitchell,1 and Joseph Heitman1,2,3,4*

Departments of Molecular Genetics and Microbiology,1 Medicine,3 Pharmacology and Cancer Biology and,4 Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina2

Received 29 October 2004/ Accepted 17 November 2004

Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to demonstrate that a chromosomal translocation and segmental duplication occurred during construction of a congenic strain pair in the fungal human pathogen Cryptococcus neoformans. Two chromosomes underwent telomere-telomere fusion, generating a dicentric chromosome that broke to produce a chromosomal translocation, forming two novel chromosomes sharing a large segmental duplication. The duplication spans 62,872 identical nucleotides and generated a second copy of 22 predicted genes, and we hypothesize that this event may have occurred during meiosis. Gene disruption studies of one embedded gene (SMG1) corroborate that this region is duplicated in an otherwise haploid genome. These findings resolve a genome project assembly anomaly and illustrate an example of rapid genome evolution in a fungal genome rich in repetitive elements.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, 322 CARL Building, Research Dr., Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-2824. Fax: (919) 684-5458. E-mail: heitm001{at}duke.edu.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, February 2005, p. 401-406, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.401-406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sun, S., Xu, J. (2007). Genetic Analyses of a Hybrid Cross Between Serotypes A and D Strains of the Human Pathogenic Fungus Cryptococcus neoformans. Genetics 177: 1475-1486 [Abstract] [Full Text]  
  • Palmer, D. A., Thompson, J. K., Li, L., Prat, A., Wang, P. (2006). Gib2, A Novel Gbeta-like/RACK1 Homolog, Functions as a Gbeta Subunit in cAMP Signaling and Is Essential in Cryptococcus neoformans. J. Biol. Chem. 281: 32596-32605 [Abstract] [Full Text]  
  • Kavanaugh, L. A., Fraser, J. A., Dietrich, F. S. (2006). Recent Evolution of the Human Pathogen Cryptococcus neoformans by Intervarietal Transfer of a 14-Gene Fragment. Mol Biol Evol 23: 1879-1890 [Abstract] [Full Text]  
  • Selmecki, A., Forche, A., Berman, J. (2006). Aneuploidy and Isochromosome Formation in Drug-Resistant Candida albicans.. Science 313: 367-370 [Abstract] [Full Text]  


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