Eukaryotic Cell
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Eukaryotic Cell, November 2005, p. 1959-1962, Vol. 4, No. 11
1535-9778/05/$08.00+0     doi:10.1128/EC.4.11.1959-1962.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The NIT1 Promoter Allows Inducible and Reversible Silencing of Centrin in Chlamydomonas reinhardtii

Bettina Koblenz and Karl-Ferdinand Lechtreck*

Botanisches Institut der Universitaet zu Koeln, 50931 Cologne, Germany

Received 25 April 2005/ Accepted 23 June 2005

An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.

* Corresponding author. Present address: Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655. Phone: 508.856.4027. Fax: 508.856.1033. E-mail: karl.lechtreck{at}umassmed.edu.

Eukaryotic Cell, November 2005, p. 1959-1962, Vol. 4, No. 11
1535-9778/05/$08.00+0     doi:10.1128/EC.4.11.1959-1962.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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