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Eukaryotic Cell, October 2005, p. 1639-1645, Vol. 4, No. 10
1535-9778/05/$08.00+0     doi:10.1128/EC.4.10.1639-1645.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rho4 GTPase Is Involved in Secretion of Glucanases during Fission Yeast Cytokinesis

Beatriz Santos,* Ana Belén Martín-Cuadrado, Carlos R. Vázquez de Aldana, Francisco del Rey, and Pilar Pérez

Instituto Microbiología-Bioquímica, Departamento de Microbiología-Genética, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007 Salamanca, Spain

Received 26 April 2005/ Accepted 19 July 2005

Rho GTPases are regulators of signaling pathways that control actin organization and cell polarity processes in all eukaryotic cells. In Schizosaccharomyces pombe, Rho4p is involved in the regulation of septum degradation during cytokinesis. Here we show that Rho4p participates in the secretion of the glucanases Eng1p and Agn1p, which are responsible for the septum degradation. First, eng1+ or agn1+ overexpression suppressed the rho4{Delta} multiseptation phenotype, and simultaneous overproduction of Rho4p and Eng1p or of Rho4p and Agn1p caused a dramatic lysis. Second, Rho4p was not necessary for Eng1p-mediated glucanase activity as measured in cell extracts; however, rho4{Delta} cells have a lower level of (1,3)-ß-D-glucanase activity in the culture medium. Additionally, Eng1- or Agn1-green fluorescent protein did not properly localize to the septum in rho4{Delta} cells grown at 37°C. There was a decreased amount of these enzymes in the cell wall and in the culture medium of rho4{Delta} cells at 37°C. These results provide evidence that Rho4p is involved in the regulation of Eng1p and Agn1p secretion during cytokinesis.

* Corresponding author. Mailing address: Instituto Microbiología-Bioquímica, Departamento de Microbiología-Genética, CSIC/Universidad de Salamanca, Campus Unamuno, 37007 Salamanca, Spain. Phone: (34) 923-121644. Fax: (34) 923-224876. E-mail: bsr{at}usal.es.

Eukaryotic Cell, October 2005, p. 1639-1645, Vol. 4, No. 10
1535-9778/05/$08.00+0     doi:10.1128/EC.4.10.1639-1645.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rasmussen, C. G., Glass, N. L. (2007). Localization of RHO-4 Indicates Differential Regulation of Conidial versus Vegetative Septation in the Filamentous Fungus Neurospora crassa. Eukaryot Cell 6: 1097-1107 [Abstract] [Full Text]  
  • Dunkler, A., Wendland, J. (2007). Candida albicans Rho-Type GTPase-Encoding Genes Required for Polarized Cell Growth and Cell Separation. Eukaryot Cell 6: 844-854 [Abstract] [Full Text]  
  • Rincon, S. A., Santos, B., Perez, P. (2006). Fission Yeast Rho5p GTPase Is a Functional Paralogue of Rho1p That Plays a Role in Survival of Spores and Stationary-Phase Cells. Eukaryot Cell 5: 435-446 [Abstract] [Full Text]  


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