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Eukaryotic Cell, December 2004, p. 1464-1475, Vol. 3, No. 6
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.6.1464-1475.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Morphogenetic Pathway of Spore Wall Assembly in Saccharomyces cerevisiae

Alison Coluccio,1 Edith Bogengruber,2 Michael N. Conrad,3 Michael E. Dresser,3 Peter Briza,2 and Aaron M. Neiman1*

Department of Biochemistry and Cell Biology, SUNY Stony Brook, Stony Brook, New York,1 Department of Molecular Biology, University of Salzburg, Salzburg, Austria,2 Program in Molecular and Cellular Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma3

Received 28 July 2004/ Accepted 10 September 2004

The Saccharomyces cerevisiae spore is protected from environmental damage by a multilaminar extracellular matrix, the spore wall, which is assembled de novo during spore formation. A set of mutants defective in spore wall assembly were identified in a screen for mutations causing sensitivity of spores to ether vapor. The spore wall defects in 10 of these mutants have been characterized in a variety of cytological and biochemical assays. Many of the individual mutants are defective in the assembly of specific layers within the spore wall, leading to arrests at discrete stages of assembly. The localization of several of these gene products has been determined and distinguishes between proteins that likely are involved directly in spore wall assembly and probable regulatory proteins. The results demonstrate that spore wall construction involves a series of dependent steps and provide the outline of a morphogenetic pathway for assembly of a complex extracellular structure.

* Corresponding author. Mailing address: Room 332, Life Sciences, SUNY Stony Brook, Stony Brook, NY 11794-5215. Phone: (631) 632-1543. Fax: (631) 632-8575. E-mail: Aaron.Neiman{at}sunysb.edu.

Eukaryotic Cell, December 2004, p. 1464-1475, Vol. 3, No. 6
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.6.1464-1475.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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