This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Patron, N. J.
Right arrow Articles by Keeling, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patron, N. J.
Right arrow Articles by Keeling, P. J.

 Previous Article  |  Next Article 

Eukaryotic Cell, October 2004, p. 1169-1175, Vol. 3, No. 5
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.5.1169-1175.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Gene Replacement of Fructose-1,6-Bisphosphate Aldolase Supports the Hypothesis of a Single Photosynthetic Ancestor of Chromalveolates{dagger}

Nicola J. Patron, Matthew B. Rogers, and Patrick J. Keeling*

Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada

Received 14 May 2004/ Accepted 30 July 2004

Plastids (photosynthetic organelles of plants and algae) are known to have spread between eukaryotic lineages by secondary endosymbiosis, that is, by the uptake of a eukaryotic alga by another eukaryote. But the number of times this has taken place is controversial. This is particularly so in the case of eukaryotes with plastids derived from red algae, which are numerous and diverse. Despite their diversity, it has been suggested that all these eukaryotes share a recent common ancestor and that their plastids originated in a single endosymbiosis, the so-called "chromalveolate hypothesis." Here we describe a novel molecular character that supports the chromalveolate hypothesis. Fructose-1,6-bisphosphate aldolase (FBA) is a glycolytic and Calvin cycle enzyme that exists as two nonhomologous types, class I and class II. Red algal plastid-targeted FBA is a class I enzyme related to homologues from plants and green algae, and it would be predicted that the plastid-targeted FBA from algae with red algal secondary endosymbionts should be related to this class I enzyme. However, we show that plastid-targeted FBA of heterokonts, cryptomonads, haptophytes, and dinoflagellates (all photosynthetic chromalveolates) are class II plastid-targeted enzymes, completely unlike those of red algal plastids. The chromalveolate enzymes form a strongly supported group in FBA phylogeny, and their common possession of this unexpected plastid characteristic provides new evidence for their close relationship and a common origin for their plastids.

* Corresponding author. Mailing address: Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, 3529-6270 University Blvd., Vancouver, British Columbia V6T 1Z4, Canada. Phone: (604) 822-4906. Fax: (604) 822-6089. E-mail: pkeeling{at}interchange.ubc.ca.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, October 2004, p. 1169-1175, Vol. 3, No. 5
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.5.1169-1175.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Burki, F., Inagaki, Y., Brate, J., Archibald, J. M., Keeling, P. J., Cavalier-Smith, T., Sakaguchi, M., Hashimoto, T., Horak, A., Kumar, S., Klaveness, D., Jakobsen, K. S., Pawlowski, J., Shalchian-Tabrizi, K. (2009). Large-Scale Phylogenomic Analyses Reveal That Two Enigmatic Protist Lineages, Telonemia and Centroheliozoa, Are Related to Photosynthetic Chromalveolates. Gen Biol Evol 2009: 231-238 [Abstract] [Full Text]  
  • Maheswari, U., Mock, T., Armbrust, E. V., Bowler, C. (2009). Update of the Diatom EST Database: a new tool for digital transcriptomics. Nucleic Acids Res 37: D1001-D1005 [Abstract] [Full Text]  
  • Frommolt, R., Werner, S., Paulsen, H., Goss, R., Wilhelm, C., Zauner, S., Maier, U. G., Grossman, A. R., Bhattacharya, D., Lohr, M. (2008). Ancient Recruitment by Chromists of Green Algal Genes Encoding Enzymes for Carotenoid Biosynthesis. Mol Biol Evol 25: 2653-2667 [Abstract] [Full Text]  
  • Slamovits, C. H., Keeling, P. J. (2008). Plastid-Derived Genes in the Nonphotosynthetic Alveolate Oxyrrhis marina. Mol Biol Evol 25: 1297-1306 [Abstract] [Full Text]  
  • Hackett, J. D., Yoon, H. S., Li, S., Reyes-Prieto, A., Rummele, S. E., Bhattacharya, D. (2007). Phylogenomic Analysis Supports the Monophyly of Cryptophytes and Haptophytes and the Association of Rhizaria with Chromalveolates. Mol Biol Evol 24: 1702-1713 [Abstract] [Full Text]  
  • Khan, H., Parks, N., Kozera, C., Curtis, B. A., Parsons, B. J., Bowman, S., Archibald, J. M. (2007). Plastid Genome Sequence of the Cryptophyte Alga Rhodomonas salina CCMP1319: Lateral Transfer of Putative DNA Replication Machinery and a Test of Chromist Plastid Phylogeny. Mol Biol Evol 24: 1832-1842 [Abstract] [Full Text]  
  • Rogers, M. B., Gilson, P. R., Su, V., McFadden, G. I., Keeling, P. J. (2007). The Complete Chloroplast Genome of the Chlorarachniophyte Bigelowiella natans: Evidence for Independent Origins of Chlorarachniophyte and Euglenid Secondary Endosymbionts. Mol Biol Evol 24: 54-62 [Abstract] [Full Text]  
  • Gould, S. B., Sommer, M. S., Kroth, P. G., Gile, G. H., Keeling, P. J., Maier, U.-G. (2006). Nucleus-to-Nucleus Gene Transfer and Protein Retargeting into a Remnant Cytoplasm of Cryptophytes and Diatoms. Mol Biol Evol 23: 2413-2422 [Abstract] [Full Text]  
  • Richards, T. A., Dacks, J. B., Campbell, S. A., Blanchard, J. L., Foster, P. G., McLeod, R., Roberts, C. W. (2006). Evolutionary origins of the eukaryotic shikimate pathway: gene fusions, horizontal gene transfer, and endosymbiotic replacements.. Eukaryot Cell 5: 1517-1531 [Abstract] [Full Text]  
  • Shalchian-Tabrizi, K., Skanseng, M., Ronquist, F., Klaveness, D., Bachvaroff, T. R., Delwiche, C. F., Botnen, A., Tengs, T., Jakobsen, K. S. (2006). Heterotachy Processes in Rhodophyte-Derived Secondhand Plastid Genes: Implications for Addressing the Origin and Evolution of Dinoflagellate Plastids. Mol Biol Evol 23: 1504-1515 [Abstract] [Full Text]  
  • Shalchian-Tabrizi, K, Eikrem, W, Klaveness, D, Vaulot, D, Minge, M.A, Le Gall, F, Romari, K, Throndsen, J, Botnen, A, Massana, R, Thomsen, H.A, Jakobsen, K.S (2006). Telonemia, a new protist phylum with affinity to chromist lineages. Proc R Soc B 273: 1833-1842 [Abstract] [Full Text]  
  • Cavalier-Smith, T. (2006). Cell evolution and Earth history: stasis and revolution. Phil Trans R Soc B 361: 969-1006 [Abstract] [Full Text]  
  • Roger, A. J, Hug, L. A (2006). The origin and diversification of eukaryotes: problems with molecular phylogenetics and molecular clock estimation. Phil Trans R Soc B 361: 1039-1054 [Abstract] [Full Text]  
  • Cavalier-Smith, T. (2006). The tiny enslaved genome of a rhizarian alga. Proc. Natl. Acad. Sci. USA 103: 9379-9380 [Full Text]  
  • Waller, R. F., Patron, N. J., Keeling, P. J. (2006). Phylogenetic history of plastid-targeted proteins in the peridinin-containing dinoflagellate Heterocapsa triquetra. Int. J. Syst. Evol. Microbiol. 56: 1439-1447 [Abstract] [Full Text]  
  • Robertson, D. L., Tartar, A. (2006). Evolution of Glutamine Synthetase in Heterokonts: Evidence for Endosymbiotic Gene Transfer and the Early Evolution of Photosynthesis. Mol Biol Evol 23: 1048-1055 [Abstract] [Full Text]  
  • Li, S., Nosenko, T., Hackett, J. D., Bhattacharya, D. (2006). Phylogenomic Analysis Identifies Red Algal Genes of Endosymbiotic Origin in the Chromalveolates. Mol Biol Evol 23: 663-674 [Abstract] [Full Text]  
  • Obornik, M., Green, B. R. (2005). Mosaic Origin of the Heme Biosynthesis Pathway in Photosynthetic Eukaryotes. Mol Biol Evol 22: 2343-2353 [Abstract] [Full Text]  
  • Bachvaroff, T. R., Sanchez Puerta, M. V., Delwiche, C. F. (2005). Chlorophyll c-Containing Plastid Relationships Based on Analyses of a Multigene Data Set with All Four Chromalveolate Lineages. Mol Biol Evol 22: 1772-1782 [Abstract] [Full Text]  
  • Montsant, A., Jabbari, K., Maheswari, U., Bowler, C. (2005). Comparative Genomics of the Pennate Diatom Phaeodactylum tricornutum. Plant Physiol. 137: 500-513 [Abstract] [Full Text]  
  • Keeling, P. J., Archibald, J. M., Fast, N. M., Palmer, J. D. (2004). Comment on "The Evolution of Modern Eukaryotic Phytoplankton". Science 306: 2191b-2191b [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»