This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bulmer, R. Articles by Morgan, B. A. Search for Related Content PubMed PubMed Citation Articles by Bulmer, R. Articles by Morgan, B. A.

 Previous Article  |  Next Article 

Eukaryotic Cell, August 2004, p. 944-954, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.944-954.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Forkhead Transcription Factor Fkh2 Regulates the Cell Division Cycle of Schizosaccharomyces pombe

Richard Bulmer,^1, Aline Pic-Taylor,^1, Simon K. Whitehall,¹ Kate A. Martin,¹ Jonathan B. A. Millar,² Janet Quinn,¹ and Brian A. Morgan^1*

Institute of Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH,¹ Division of Yeast Genetics, National Institute for Medical Research, London NW7 1AA, United Kingdom²

Received 27 January 2004/ Accepted 27 April 2004

In eukaryotes the regulation of gene expression plays a key role in controlling cell cycle progression. Here, we demonstrate that a forkhead transcription factor, Fkh2, regulates the periodic expression of cdc15⁺ and spo12⁺ in the M and G₁ phases of the cell division cycle in the fission yeast Schizosaccharomyces pombe. We also show that Fkh2 is important for several cell cycle processes, including cell morphology and cell separation, nuclear structure and migration, and mitotic spindle function. We find that the expression of fkh2⁺ is itself regulated in a cell cycle-dependent manner in G₁ coincident with the expression of cdc18⁺, a Cdc10-regulated gene. However, fkh2⁺ expression is independent of Cdc10 function. Fkh2 was found to be phosphorylated during the cell division cycle, with a timing that suggests that this posttranslational modification is important for cdc15⁺ and spo12⁺ expression. Related forkhead proteins regulate G₂ and M phase-specific gene expression in the evolutionarily distant Saccharomyces cerevisiae, suggesting that these proteins play conserved roles in regulating cell cycle processes in eukaryotes.

---

* Corresponding author. Mailing address: Institute of Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom. Phone: 0044-191 222 7694. Fax: 0044-191 222 7424. E-mail: b.a.morgan{at}ncl.ac.uk.

R.B. and A.P.-T. contributed equally to this work.

---

Eukaryotic Cell, August 2004, p. 944-954, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.944-954.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Papadopoulou, K., Ng, S. S., Ohkura, H., Geymonat, M., Sedgwick, S. G., McInerny, C. J. (2008). Regulation of gene expression during M-G1-phase in fission yeast through Plo1p and forkhead transcription factors. J. Cell Sci. 121: 38-47 [Abstract] [Full Text]  
• Sherriff, J. A., Kent, N. A., Mellor, J. (2007). The Isw2 Chromatin-Remodeling ATPase Cooperates with the Fkh2 Transcription Factor To Repress Transcription of the B-Type Cyclin Gene CLB2. Mol. Cell. Biol. 27: 2848-2860 [Abstract] [Full Text]  
• Nolting, N., Poggeler, S. (2006). A MADS Box Protein Interacts with a Mating-Type Protein and Is Required for Fruiting Body Development in the Homothallic Ascomycete Sordaria macrospora.. Eukaryot Cell 5: 1043-1056 [Abstract] [Full Text]  
• Petit, C. S., Mehta, S., Roberts, R. H., Gould, K. L. (2005). Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression. J. Cell Sci. 118: 5731-5742 [Abstract] [Full Text]