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Eukaryotic Cell, February 2004, p. 52-60, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.52-60.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

C-Terminal Truncation of {alpha}-COP Affects Functioning of Secretory Organelles and Calcium Homeostasis in Hansenula polymorpha

Maria B. Chechenova, Nina V. Romanova, Alexander V. Deev, Anna N. Packeiser, Vladimir N. Smirnov, Michael O. Agaphonov,* and Michael D. Ter-Avanesyan

Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia

Received 19 July 2003/ Accepted 27 October 2003

In eukaryotic cells, COPI vesicles retrieve resident proteins to the endoplasmic reticulum and mediate intra-Golgi transport. Here, we studied the Hansenula polymorpha homologue of the Saccharomyces cerevisiae RET1 gene, encoding {alpha}-COP, a subunit of the COPI protein complex. H. polymorpha ret1 mutants, which expressed truncated {alpha}-COP lacking more than 300 C-terminal amino acids, manifested an enhanced ability to secrete human urokinase-type plasminogen activator (uPA) and an inability to grow with a shortage of Ca2+ ions, whereas a lack of {alpha}-COP expression was lethal. The {alpha}-COP defect also caused alteration of intracellular transport of the glycosylphosphatidylinositol-anchored protein Gas1p, secretion of abnormal uPA forms, and reductions in the levels of Pmr1p, a Golgi Ca2+-ATPase. Overexpression of Pmr1p suppressed some ret1 mutant phenotypes, namely, Ca2+ dependence and enhanced uPA secretion. The role of COPI-dependent vesicular transport in cellular Ca2+ homeostasis is discussed.

* Corresponding author. Mailing address: Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya St. 15A, 121552 Moscow, Russia. Phone: (7-095) 414-67-38. Fax: (7-095) 414-67-33. E-mail: aga{at}cardio.ru.

Eukaryotic Cell, February 2004, p. 52-60, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.52-60.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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