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 Previous Article

Eukaryotic Cell, February 2004, p. 241-244, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.241-244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SmD1 Is Required for Spliced Leader RNA Biogenesis

Gusti M. Zeiner,1,{dagger} Silvie Foldynová,2,{dagger} Nancy R. Sturm,1 Julius Lukes,2 and David A. Campbell1*

Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095-1489,1 Institute of Parasitology, Czech Academy of Sciences, and Faculty of Biology, University of South Bohemia, Ceské Budejovice, Czech Republic2

Received 22 August 2003/ Accepted 10 December 2003

The Sm-binding site of the kinetoplastid spliced leader RNA has been implicated in accurate spliced leader RNA maturation and trans-splicing competence. In Trypanosoma brucei, RNA interference-mediated knockdown of SmD1 caused defects in spliced leader RNA maturation, displaying aberrant 3'-end formation, partial formation of cap 4, and overaccumulation in the cytoplasm; U28 pseudouridylation was unaffected.

* Corresponding author. Mailing address: Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine at UCLA, University of California at Los Angeles, 609 Charles E. Young Dr. East, Los Angeles, CA 90095-1489. Phone: (310) 206-5556. Fax: (310) 206-5231. E-mail: dc{at}ucla.edu.

{dagger} G.M.Z. and S.F. contributed equally to this work.

Eukaryotic Cell, February 2004, p. 241-244, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.241-244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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