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Eukaryotic Cell, February 2004, p. 144-156, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.144-156.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Chromatin Rearrangements in the prnD-prnB Bidirectional Promoter: Dependence on Transcription Factors

Irene García,1,{dagger}* Ramón Gonzalez,1,{ddagger} Dennis Gómez,1,§ and Claudio Scazzocchio1,2

Institut de Génétique et Microbiologie,1 Institut Universitaire de France, Université Paris-Sud, UMR8621, 91405 Orsay Cedex, France2

Received 20 November 2003/ Accepted 24 November 2003

The prnD-prnB intergenic region regulates the divergent transcription of the genes encoding proline oxidase and the major proline transporter. Eight nucleosomes are positioned in this region. Upon induction, the positioning of these nucleosomes is lost. This process depends on the specific transcriptional activator PrnA but not on the general GATA factor AreA. Induction of prnB but not prnD can be elicited by amino acid starvation. A specific nucleosomal pattern in the prnB proximal region is associated with this process. Under conditions of induction by proline, metabolite repression depends on the presence of both repressing carbon (glucose) and nitrogen (ammonium) sources. Under these repressing conditions, partial nucleosomal positioning is observed. This depends on the CreA repressor's binding to two specific cis-acting sites. Three conditions (induction by the defective PrnA80 protein, induction by amino acid starvation, and induction in the presence of an activated CreA) result in similar low transcriptional activation. Each results in a different nucleosome pattern, which argues strongly for a specific effect of each signal on nucleosome positioning. Experiments with trichostatin A suggest that both default nucleosome positioning and partial positioning under induced-repressed conditions depend on deacetylated histones.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis (CSIC), Centro de Investigaciones Científicas de la Isla de la Cartuja, Avda. Americo Vespucio s/n, 41092 Seville, Spain. Phone: 34 954489516. Fax: 34 954460065. E-mail: irene.garcia{at}ibvf.csic.es.

{dagger} Present address: Instituto de Bioquímica Vegetal y Fotosíntesis (CSIC), Centro de Investigaciones Científicas de la Isla de la Cartuja, 41092 Seville, Spain.

{ddagger} Present address: Instituto de Fermentaciones Industriales (CSIC), C/ Juan de la Cierva 3, 28006 Madrid, Spain.

§ Present address: Laboratoire de Physiologie Humaine, Faculté de Pharmacie-Médecine, 51100 Reims, France.

Eukaryotic Cell, February 2004, p. 144-156, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.144-156.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Reyes-Dominguez, Y., Narendja, F., Berger, H., Gallmetzer, A., Fernandez-Martin, R., Garcia, I., Scazzocchio, C., Strauss, J. (2008). Nucleosome Positioning and Histone H3 Acetylation Are Independent Processes in the Aspergillus nidulans prnD-prnB Bidirectional Promoter. Eukaryot Cell 7: 656-663 [Abstract] [Full Text]  
  • Byrnes, J. K., Morris, G. P., Li, W.-H. (2006). Reorganization of Adjacent Gene Relationships in Yeast Genomes by Whole-Genome Duplication and Gene Deletion. Mol Biol Evol 23: 1136-1143 [Abstract] [Full Text]  
  • Muro-Pastor, M. I., Strauss, J., Ramon, A., Scazzocchio, C. (2004). A Paradoxical Mutant GATA Factor. Eukaryot Cell 3: 393-405 [Abstract] [Full Text]  


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