This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, F.
Right arrow Articles by Palecek, S. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, F.
Right arrow Articles by Palecek, S. P.

 Previous Article  |  Next Article 

Eukaryotic Cell, December 2003, p. 1266-1273, Vol. 2, No. 6
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.6.1266-1273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

EAP1, a Candida albicans Gene Involved in Binding Human Epithelial Cells

Fang Li and Sean P. Palecek*

Department of Chemical and Biological Engineering, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 10 June 2003/ Accepted 2 October 2003

Candida albicans adhesion to host tissues contributes to its virulence and adhesion to medical devices permits biofilm formation, but we know relatively little about the molecular mechanisms governing C. albicans adhesion to materials or mammalian cells. Saccharomyces cerevisiae provides an attractive model system for studying adhesion in yeast because of its well-characterized genetics and gene expression systems and the conservation of signal transduction pathways among the yeasts. In this study, we used a parallel plate flow chamber to screen and characterize attachment of a flo8{Delta} S. cerevisiae strain expressing a C. albicans genomic library to a polystyrene surface. The gene EAP1 was isolated as a putative cell wall adhesin. Sequence analysis of EAP1 shows that it contains a signal peptide, a glycosylphosphatidylinositol anchor site, and possesses homology to many other yeast genes encoding cell wall proteins. In addition to increasing adhesion to polystyrene, heterologous expression of EAP1 in S. cerevisiae and autonomous expression of EAP1 in a C. albicans efg1 homozygous null mutant significantly enhanced attachment to HEK293 kidney epithelial cells. EAP1 expression also restored invasive growth to haploid flo8{Delta} and flo11{Delta} strains as well as filamentous growth to diploid flo8/flo8 and flo11/flo11 strains. Transcription of EAP1 in C. albicans is regulated by the transcription factor Efg1p, suggesting that EAP1 expression is activated by the cyclic AMP-dependent protein kinase pathway.

* Corresponding author. Mailing address: Department of Chemical and Biological Engineering, 1415 Engineering Dr., Madison, WI 53706. Phone: (608) 262-8931. Fax: (608) 262-5434. E-mail: palecek{at}engr.wisc.edu.

Eukaryotic Cell, December 2003, p. 1266-1273, Vol. 2, No. 6
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.6.1266-1273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moreno-Ruiz, E., Ortu, G., de Groot, P. W. J., Cottier, F., Loussert, C., Prevost, M.-C., de Koster, C., Klis, F. M., Goyard, S., d'Enfert, C. (2009). The GPI-modified proteins Pga59 and Pga62 of Candida albicans are required for cell wall integrity. Microbiology 155: 2004-2020 [Abstract] [Full Text]  
  • Huang, G., Dougherty, S. D., Erdman, S. E. (2009). Conserved WCPL and CX4C Domains Mediate Several Mating Adhesin Interactions in Saccharomyces cerevisiae. Genetics 182: 173-189 [Abstract] [Full Text]  
  • ten Cate, J.M., Klis, F.M., Pereira-Cenci, T., Crielaard, W., de Groot, P.W.J. (2009). Molecular and Cellular Mechanisms That Lead to Candida Biofilm Formation. JDR 88: 105-115 [Abstract] [Full Text]  
  • de Groot, P. W. J., Kraneveld, E. A., Yin, Q. Y., Dekker, H. L., Gross, U., Crielaard, W., de Koster, C. G., Bader, O., Klis, F. M., Weig, M. (2008). The Cell Wall of the Human Pathogen Candida glabrata: Differential Incorporation of Novel Adhesin-Like Wall Proteins. Eukaryot Cell 7: 1951-1964 [Abstract] [Full Text]  
  • Chaffin, W. L. (2008). Candida albicans Cell Wall Proteins. Microbiol. Mol. Biol. Rev. 72: 495-544 [Abstract] [Full Text]  
  • Li, F., Palecek, S. P. (2008). Distinct domains of the Candida albicans adhesin Eap1p mediate cell-cell and cell-substrate interactions. Microbiology 154: 1193-1203 [Abstract] [Full Text]  
  • Douglas, L. M., Li, L., Yang, Y., Dranginis, A. M. (2007). Expression and Characterization of the Flocculin Flo11/Muc1, a Saccharomyces cerevisiae Mannoprotein with Homotypic Properties of Adhesion. Eukaryot Cell 6: 2214-2221 [Abstract] [Full Text]  
  • Norice, C. T., Smith, F. J. Jr., Solis, N., Filler, S. G., Mitchell, A. P. (2007). Requirement for Candida albicans Sun41 in Biofilm Formation and Virulence. Eukaryot Cell 6: 2046-2055 [Abstract] [Full Text]  
  • Dranginis, A. M., Rauceo, J. M., Coronado, J. E., Lipke, P. N. (2007). A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial Occasions. Microbiol. Mol. Biol. Rev. 71: 282-294 [Abstract] [Full Text]  
  • Li, F., Svarovsky, M. J., Karlsson, A. J., Wagner, J. P., Marchillo, K., Oshel, P., Andes, D., Palecek, S. P. (2007). Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation In Vitro and In Vivo. Eukaryot Cell 6: 931-939 [Abstract] [Full Text]  
  • Alvarez, F. J., Douglas, L. M., Konopka, J. B. (2007). Sterol-Rich Plasma Membrane Domains in Fungi. Eukaryot Cell 6: 755-763 [Full Text]  
  • Albrecht, A., Felk, A., Pichova, I., Naglik, J. R., Schaller, M., de Groot, P., MacCallum, D., Odds, F. C., Schafer, W., Klis, F., Monod, M., Hube, B. (2006). Glycosylphosphatidylinositol-anchored Proteases of Candida albicans Target Proteins Necessary for Both Cellular Processes and Host-Pathogen Interactions. J. Biol. Chem. 281: 688-694 [Abstract] [Full Text]  
  • Cho, J., Degen, J. L., Coller, B. S., Mosher, D. F. (2005). Fibrin but Not Adsorbed Fibrinogen Supports Fibronectin Assembly by Spread Platelets: EFFECTS OF THE INTERACTION OF {alpha}IIb{beta}3 WITH THE C TERMINUS OF THE FIBRINOGEN {gamma}-CHAIN. J. Biol. Chem. 280: 35490-35498 [Abstract] [Full Text]  
  • Rouabhia, M., Schaller, M., Corbucci, C., Vecchiarelli, A., Prill, S. K.-H., Giasson, L., Ernst, J. F. (2005). Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases. Infect. Immun. 73: 4571-4580 [Abstract] [Full Text]  
  • Ramage, G., Saville, S. P., Thomas, D. P., Lopez-Ribot, J. L. (2005). Candida Biofilms: an Update. Eukaryot Cell 4: 633-638 [Full Text]  
  • Weig, M., Jansch, L., Gross, U., De Koster, C. G., Klis, F. M., De Groot, P. W. J. (2004). Systematic identification in silico of covalently bound cell wall proteins and analysis of protein-polysaccharide linkages of the human pathogen Candida glabrata. Microbiology 150: 3129-3144 [Abstract] [Full Text]  
  • Rauceo, J. M., Gaur, N. K., Lee, K.-G., Edwards, J. E., Klotz, S. A., Lipke, P. N. (2004). Global Cell Surface Conformational Shift Mediated by a Candida albicans Adhesin. Infect. Immun. 72: 4948-4955 [Abstract] [Full Text]  
  • de Groot, P. W. J., de Boer, A. D., Cunningham, J., Dekker, H. L., de Jong, L., Hellingwerf, K. J., de Koster, C., Klis, F. M. (2004). Proteomic Analysis of Candida albicans Cell Walls Reveals Covalently Bound Carbohydrate-Active Enzymes and Adhesins. Eukaryot Cell 3: 955-965 [Abstract] [Full Text]  
  • Sheppard, D. C., Yeaman, M. R., Welch, W. H., Phan, Q. T., Fu, Y., Ibrahim, A. S., Filler, S. G., Zhang, M., Waring, A. J., Edwards, J. E. Jr. (2004). Functional and Structural Diversity in the Als Protein Family of Candida albicans. J. Biol. Chem. 279: 30480-30489 [Abstract] [Full Text]  
  • Martin, S. W., Konopka, J. B. (2004). Lipid Raft Polarization Contributes to Hyphal Growth in Candida albicans. Eukaryot Cell 3: 675-684 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»