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Eukaryotic Cell, October 2003, p. 901-909, Vol. 2, No. 5
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.5.901-909.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Conservation of RNase III Processing Pathways and Specificity in Hemiascomycetes

Guillaume Chanfreau*

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095-1569

Received 13 May 2003/ Accepted 3 July 2003

Rnt1p, the only known Saccharomyces cerevisiae RNase III endonuclease, plays important functions in the processing of precursors of rRNAs (pre-rRNAs) and of a large number of small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs). While most eukaryotic RNases III, including the Schizosaccharomyces pombe enzyme Pac1p, cleave double-stranded RNA without sequence specificity, Rnt1p cleavage relies on the presence of terminal tetraloop structures that carry the consensus sequence AGNN. To search for the conservation of these processing signals, I have systematically analyzed predicted secondary structures of the 3' external transcribed spacer (ETS) sequences of the pre-rRNAs and of flanking sequences of snRNAs and snoRNAs from sequences available in 13 other Hemiascomycetes species. In most of these species, except in Yarrowia lipolytica, double-stranded RNA regions capped by terminal AGNN tetraloops can be found in the 3' ETS sequences of rRNA, in the 5'- or 3'-end flanking sequences of sn(o)RNAs, or in the intergenic spacers of polycistronic snoRNA transcription units. This analysis shows that RNase III processing signals and RNase III cleavage specificity are conserved in most Hemiascomycetes species but probably not in the evolutionarily more distant species Y. lipolytica.

* Mailing address: Department of Chemistry and Biochemistry, University of California Los Angeles, 607 Charles E. Young Dr. East, Box 951569, Los Angeles, CA 90095. Phone: (310) 825-4399. Fax: (310) 206-4038. E-mail: guillom{at}chem.ucla.edu.

Eukaryotic Cell, October 2003, p. 901-909, Vol. 2, No. 5
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.5.901-909.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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