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Eukaryotic Cell, June 2003, p. 431-445, Vol. 2, No. 3
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.3.431-445.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Ady4p and Spo74p Are Components of the Meiotic Spindle Pole Body That Promote Growth of the Prospore Membrane in Saccharomyces cerevisiae

Mark E. Nickas,1 Cindi Schwartz,2 and Aaron M. Neiman1*

Department of Biochemistry and Cell Biology and Institute for Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, New York 11794-5215,1 Boulder Laboratory for 3-D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 803092

Received 30 January 2003/ Accepted 28 March 2003

Spore formation in Saccharomyces cerevisiae occurs via the de novo synthesis of the prospore membrane during the second meiotic division. Prospore membrane formation is triggered by assembly of a membrane-organizing center, the meiotic outer plaque (MOP), on the cytoplasmic face of the spindle pole body (SPB) during meiosis. We report here the identification of two new components of the MOP, Ady4p and Spo74p. Ady4p and Spo74p interact with known proteins of the MOP and are localized to the outer plaque of the SPB during meiosis II. MOP assembly and prospore membrane formation are abolished in spo74{Delta}/spo74{Delta} cells and occur aberrantly in ady4{Delta}/ady4{Delta} cells. Spo74p and the MOP component Mpc70p are mutually dependent for recruitment to SPBs during meiosis. In contrast, both Ady4p and Spo74p are present at SPBs, albeit at reduced levels, in cells that lack the MOP component Mpc54p. Our findings suggest a model for the assembled MOP in which Mpc54p, Mpc70p, and Spo74p make up a core structural unit of the scaffold that initiates synthesis of the prospore membrane, and Ady4p is an auxiliary component that stabilizes the plaque.

* Corresponding author. Mailing address: 332 Life Sciences, Department of Biochemistry and Cell Biology, SUNY Stony Brook, Stony Brook, NY 11794-5215. Phone: (631) 632-1543. Fax: (631) 632-8575. E-mail: Aaron.Neiman{at}sunysb.edu.

Eukaryotic Cell, June 2003, p. 431-445, Vol. 2, No. 3
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.3.431-445.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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