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Eukaryotic Cell, April 2003, p. 341-350, Vol. 2, No. 2
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.2.341-350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Histone H1 Is Required for Proper Regulation of Pyruvate Decarboxylase Gene Expression in Neurospora crassa

H. Diego Folco,1 Michael Freitag,2 Ana Ramón,3 Esteban D. Temporini,4,{dagger} María E. Alvarez,4 Irene García,3 Claudio Scazzocchio,3 Eric U. Selker,2 and Alberto L. Rosa1*

Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET,1 Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 5016 Córdoba, Argentina,4 Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229,2 Institut de Génétique et Microbiologie, Université de Paris-Sud, UMR 8621, 91405 Orsay Cedex, France3

Received 31 July 2002/ Accepted 20 December 2002

We show that Neurospora crassa has a single histone H1 gene, hH1, which encodes a typical linker histone with highly basic N- and C-terminal tails and a central globular domain. A green fluorescent protein-tagged histone H1 chimeric protein was localized exclusively to nuclei. Mutation of hH1 by repeat-induced point mutation (RIP) did not result in detectable defects in morphology, DNA methylation, mutagen sensitivity, DNA repair, fertility, RIP, chromosome pairing, or chromosome segregation. Nevertheless, hH1 mutants had mycelial elongation rates that were lower than normal on all tested carbon sources. This slow linear growth phenotype, however, was less evident on medium containing ethanol. The pyruvate decarboxylase gene, cfp, was abnormally derepressed in hH1 mutants on ethanol-containing medium. This derepression was also found when an ectopically integrated fusion of the cfp gene promoter to the reporter gene hph was analyzed. Thus, Neurospora histone H1 is required for the proper regulation of cfp, a gene with a key role in the respiratory-fermentative pathway.

* Corresponding author. Mailing address: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET, Friuli 2434, 5016 Córdoba, Argentina. Phone: (54)(351)468-1465. Fax: (54)(351)469-5163. E-mail: arosa{at}immf.uncor.edu.

{dagger} Present address: Department of Plant Pathology, University of Arizona, Tucson, AZ 85721.

Eukaryotic Cell, April 2003, p. 341-350, Vol. 2, No. 2
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.2.341-350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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