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Eukaryotic Cell, February 2003, p. 103-114, Vol. 2, No. 1
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.1.103-114.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Tec3, a New Developmentally Eliminated DNA Element in Euplotes crassus

Mary Ellen Jacobs,1* Adolfo Sánchez-Blanco,1 Laura A. Katz,2 and Lawrence A. Klobutcher1

Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030,1 Department of Biological Sciences, Smith College, Northampton, Massachusetts 010632

Received 20 June 2002/ Accepted 23 September 2002

More than 100,000 interstitial segments of DNA (internal eliminated sequences [IESs]) are excised from the genome during the formation of a new macronucleus in Euplotes crassus. IESs include unique sequence DNA as well as two related families of transposable elements, Tec1 and Tec2. Here we describe a new class of E. crassus transposons, Tec3, which is present in 20 to 30 copies in the micronuclear genome. Tec3 elements have long inverted terminal repeats and contain a degenerate open reading frame encoding a tyrosine-type recombinase. One characterized copy of Tec3 (Tec3-1) is 4.48 kbp long, has 1.23-kbp inverted terminal repeats, and resides within the micronuclear copy of the ribosomal protein L29 gene (RPL29). The 23 bp at the extreme ends of this element are very similar to those in other E. crassus IESs and, like these other IESs, Tec3-1 is excised during the polytene chromosome stage of macronuclear development to generate a free circular form with an unusual junction structure. In contrast, a second cloned element, Tec3-2, is quite similar to Tec3-1 but lacks the terminal 258 bp of the inverted repeats, so that its ends do not resemble the other E. crassus IES termini. The Tec3-2 element appears to reside in a large segment of the micronuclear genome that is subject to developmental elimination. Models for the origins of these two types of Tec3 elements are presented, along with a discussion of how some members of this new transposon family may have come to be excised by the same machinery that removes other E. crassus IESs.

* Corresponding author. Mailing address: Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030. Phone: (860) 679-2224. Fax: (860) 679-3408. E-mail: jacobs{at}neuron.uchc.edu.

Eukaryotic Cell, February 2003, p. 103-114, Vol. 2, No. 1
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.1.103-114.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Goodwin, T. J. D., Butler, M. I., Poulter, R. T. M. (2003). Cryptons: a group of tyrosine-recombinase-encoding DNA transposons from pathogenic fungi. Microbiology 149: 3099-3109 [Abstract] [Full Text]  
  • Doak, T. G., Witherspoon, D. J., Jahn, C. L., Herrick, G. (2003). Selection on the Genes of Euplotes crassus Tec1 and Tec2 Transposons: Evolutionary Appearance of a Programmed Frameshift in a Tec2 Gene Encoding a Tyrosine Family Site-Specific Recombinase. Eukaryot Cell 2: 95-102 [Abstract] [Full Text]  


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