Files in this Data Supplement:

• Supplemental file 1 - Table S1. Potential interactors for Rho1 and Pdc1.
  MS Word document, 77K.
• Supplemental file 2 - Supplemental figure legends.
  MS Word document, 33.5K.
• Supplemental file 3 - Fig. S1. RNA expression levels of induced and repressed rho1^crg1 in Δrac1-background cells.
  TIFF file, 54.7K.
• Supplemental file 4 - Fig. S2. Phenotypes of rho1^crg1 cells growing on arabinose medium (YPA) are comparable to wild-type cells.
  TIFF file, 9.39 MB.
• Supplemental file 5 - Fig. S3. Monitoring the growth rate and rho1 transcriptional activity of pUM-rho1 cells.
  TIFF file, 12.3 MB.
• Supplemental file 6 - Fig. S4. Coimmunoprecipitation to test interactions between Rho1 and potential interactors.
  TIFF file, 14.5 MB.
• Supplemental file 7 - Fig. S5. pdc1 partially complements yeast Δbmh1 bmh2 mutant.
  TIFF file, 3.93 MB.
• Supplemental file 8 - Fig. S6. Pdc1 transiently localizes at the growing tip.
  TIFF file, 3.51 MB.
• Supplemental file 9 - Fig. S7. Steady-state RNA levels of induced and repressed pdc1^crg1 cells.
  TIFF file, 530K.
• Supplemental file 10 - Fig. S8. Phenotype of pdc1^crg1 cells growing on arabinose medium (YPA) are similar to wild-type cells.
  TIFF file, 9.74 MB.
• Supplemental file 11 - Fig. S9. Steady-state RNA levels of induced and repressed pdc1^nar1 in Δrac1 and Δcla4 background cells.
  TIFF file, 1 MB.