Eukaryotic Cell, June 2009, p. 805, Vol. 8, No. 6
1535-9778/09/$08.00+0 doi:10.1128/EC.00134-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
| SPOTLIGHT |
Articles of Significant Interest Selected from This Issue by the Editors
Extending the Reach of Candida albicans Cap1pGenome-wide location profiling (also known as ChIP-on-chip) is a powerful tool to identify transcription factor binding sites and, combined with transcription profiling, provides detailed insights into the regulatory networks controlling gene expression. Znaidi et al. (p. 806-820) applied this technology to the Cap1p transcription factor from Candida albicans, an important human fungal pathogen in terms of both its clinical significance and its use as an experimental model for fungal pathogenesis investigation. The Cap1p regulon was found to include genes involved in oxidative stress response, azole drug resistance, nitrogen metabolism, and phospholipid transport, demonstrating that Cap1p regulates different cellular processes in C. albicans. High-density oligonucleotide tiling microarrays allowed the detection of Cap1p binding at the promoter region of its target genes but also at their 3' ends and within their open reading frames, suggesting that Cap1p associates with the transcriptional or the chromatin remodeling machinery to exert its activity. This study exemplifies the strong discovery power of genome-wide location and expression profiling analyses and represents a major step forward in our understanding of the transcriptional regulatory networks in C. albicans.
Histone Deacetylation Complex Component Linked to Pathogenicity
The gene encoding FTL1, a transducin beta-like protein from Fusarium graminearum, was identified in an insertional mutagenesis screen and found to encode a protein homologous to yeast SIF2, a component of the Set3 complex associated with both histone deacetylation and gene repression by means of chromatin modification. ftl1 deletion mutants had multiple phenotypes, including reduced conidiation, slow growth, and reduced pathogenicity on wheat. The data reported by Ding et al. (p. 867-876) are novel and of potential interest for the scientific community, since this is the first report linking a component of the Set3 protein complex to fungal pathogenicity on plants.
Eukaryotic Cell, June 2009, p. 805, Vol. 8, No. 6
1535-9778/09/$08.00+0 doi:10.1128/EC.00134-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
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