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Eukaryotic Cell, February 2006, p. 321-329, Vol. 5, No. 2
1535-9778/06/$08.00+0     doi:10.1128/EC.5.2.321-329.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rac1 and Cdc42 Have Different Roles in Candida albicans Development

Martine Bassilana^* and Robert A. Arkowitz

Institute of Signaling, Developmental Biology, and Cancer, CNRS UMR 6543, Université de Nice, Faculté des Sciences-Parc Valrose, 06108 Nice Cedex 2, France

Received 16 October 2005/ Accepted 28 November 2005

Top Abstract Introduction Materials and Methods Results Discussion References

 
We investigated the role of the highly conserved G protein Rac1 in the opportunistic pathogen Candida albicans. We identified and disrupted RAC1 and show here that, in contrast to CDC42, it is not necessary for viability or serum-induced hyphal growth but is essential for filamentous growth when cells are embedded in a matrix. Rac1 is localized to the plasma membrane, yet its distribution is more homogenous than that of Cdc42, with no enrichment at the tips of either buds or hyphae. In addition, fluorescence recovery after photobleaching results indicate that Rac1 and Cdc42 have different dynamics at the membrane. Furthermore, overexpression of Rac1 does not complement Cdc42 function, and conversely, overexpression of Cdc42 does not complement Rac1 function. Thus, Rac1 and Cdc42, although highly similar to one another, have different roles in C. albicans development.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Candida albicans is an opportunistic human fungal pathogen which causes essentially superficial mycoses of skin or the mucosal epithelia (20) but also can be life-threatening to immunodeficient patients (15). C. albicans is a dimorphic fungus, i.e., it can switch from budding to filamentous growth, and this dimorphic transition is important for its pathogenicity (10, 26). In order to restrict the polarized growth necessary for invasive C. albicans filamentous development, cells need to reorganize their cytoskeleton.

Rho-type G proteins are required for cytoskeleton organization in virtually all eukaryotic cells (13). They are divided into three subfamilies, Rho, Rac, and Cdc42, within the Ras superfamily. In mammals, Rac and Cdc42, in particular, play overlapping and complementary functions in cell morphogenesis, division, and migration (13). In fungi, the functions of Cdc42 and Rac1 appear to vary. For example, in Yarrowia lipolytica, Rac1 is not essential for cell viability or actin organization but is required for hyphal growth (12). In contrast, in Penicillium marneffei, the Rac homolog CflB plays a crucial role in actin cytoskeleton organization and vegetative growth (5). In Cryptococcus neoformans, Rac1 is not required for actin cytoskeleton organization but is required for haploid filamentation and mating (22). In the ascomycete Saccharomyces cerevisiae, Cdc42 is required for viability (14), mating (19), and pseudohyphal development (18); however, no Rac1 ortholog exists.

In C. albicans, Cdc42 and its exchange factor, Cdc24, have critical roles in viability, the bud-to-hypha transition, hyphal growth maintenance, and pathogenicity in a murine model of systemic candidiasis (3, 4, 17, 21, 23). Here we identify a Rac1 protein in C. albicans which has 60% overall sequence identity with its human counterpart and 57% identity with C. albicans Cdc42. We have disrupted RAC1 and show here that this G protein is not necessary for viability or actin cytoskeleton organization. Rac1 is essential for filamentous growth when cells are embedded in an agar matrix yet is not required for serum-induced hyphal growth, in contrast to Cdc42. We also show that Rac1 is localized to the plasma membrane with different dynamics from those of Cdc42. Furthermore, overexpression of Rac1 does not complement Cdc42 functions. Altogether, our results indicate that although they are highly similar to one another, Rac1 and Cdc42 have fundamentally different roles in C. albicans development.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Strain construction. The strains and primers used for this study are listed in Tables 1 and 2, respectively. The rac1/RAC1 mutant (PY156) was obtained by transforming an auxotrophic his^– ura^– arg^– BPW17 strain (27) with a rac1-1::URA3 PCR product generated with the RAC1.P1 and RAC1.P2 primers and pGemUra3. This deletion removed the majority of the RAC1 open reading frame (ORF), leaving 44 bp 3' of the ATG and 30 bp 5' of the stop codon. A rac1-2::HIS1 cassette was generated by first cloning RAC1 using the RAC1.P3 and -P4 primers into pCR2.1-TOPO (Invitrogen) and then inserting a BamHI-XbaI-blunted HIS1 gene fragment from pGemHis1. This rac1-2::HIS1 cassette was transformed into PY156, and URA⁺ HIS⁺ prototrophs were selected. This second deletion also removed the majority of the RAC1 ORF, leaving 28 bp 3' of the ATG and 32 bp 5' of the stop codon. pExpArg constructs were digested with StuI and targeted to the RP10 locus in the appropriate strains.

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TABLE 1. Yeast strains used for this study

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TABLE 2. Primers used for this study

Initially, the RAC1 ORF, with 677 bp 5' of the ATG and 406 bp 3' of the stop codon (approximately 1.7 kb), was amplified from genomic DNA by PCR using primers RAC1.P7 and -P8, with unique BamHI and NotI sites at the 5' and 3' ends, respectively, and cloned into pCR2.1-TOPO. Subsequently, a BamHI/XhoI PRAC1RAC1 fragment was cloned into the respective sites in pExpArg (3). Overexpression constructs were generated by cloning PADH-RAC1-ADHT, PADH-GFP-RAC1-ADHT, and PADH-GFP-CDC42-ADHT into pExpArg. A PCR-amplified RAC1 ORF with a BamHI site 5' of the ATG and an MluI site 3' of the stop codon (generated using primers RAC1.P9 and -P13) was cloned into the BglII and MluI sites of pAW6-X (4), yielding pAW6-X-RAC1. yeGFP was amplified using primers GFP.P1 and -P2, with a unique BamHI site 5' of the ATG and a BglII site replacing the stop codon, respectively. This PCR product was cloned into pAW6-X (4) digested with BglII, yielding pAW6-X-GFP. A PCR-amplified RAC1 or CDC42 ORF with a BamHI site 5' of the ATG and an MluI site 3' of the stop codon (generated using primers RAC1.P9 and -P10 or CDC42.P1 and -P2) was cloned into the BglII and MluI sites of pAW6-X-GFP, yielding pAW6-X-GFP-RAC1 or pAW6-X-GFP-CDC42, respectively. NotI/XhoI PADH-RAC1-ADHT, PADH-GFP-RAC1-ADHT, and PADH-GFP-CDC42-ADHT fragments were then cloned into pExpArg, resulting in pExp-PADHRAC1, pExp-PADHGFPRAC1, and pExp-PADHGFPCDC42, respectively. The StuI site in the CDC42 ORF was removed by site-directed mutagenesis. Dominant active (G12V) and dominant negative (T17N) versions of Rac1 were generated by site-directed mutagenesis of pExp-PADHGFPRAC1. All constructs were verified by dye terminator sequencing (ABI Prism).

Growth conditions. Cells were grown at 30°C in yeast extract-peptone-dextrose (YEPD) medium unless indicated otherwise. Serum induction was carried out as described previously (3), using overnight cultures grown in YEPD that were back diluted in YEPD and grown for approximately 6 h prior to the addition of 50% serum. Embedded medium conditions were as described previously (6), using YEP and sucrose.

Microscopy. Colonies and cells were imaged as described previously (3). The actin cytoskeleton was visualized as described previously (19), except that Alexa 488-phalloidin (Molecular Probes) was used and images were taken as described elsewhere (2). Fluorescence recovery after photobleaching (FRAP) analysis was performed on a Zeiss LSM510 Meta inverted confocal microscope using a Plan-Apo 63x (numerical aperture, 1.4) objective. Images were captured every 0.4 second at 1 or 2% maximum laser intensity. Bleaching was performed at 90% laser intensity using 10 x 0.6-ms photobleaching scans on a bud or hyphal tip circular area of 1.1 µm². Data analysis was done with Excel (Microsoft), and the average intensity of the bleached area was normalized to photobleaching during image acquisition, using the average intensity of an adjacent cell. Regression analysis to determine the FRAP t_1/2 was done using a one-phase exponential association function in SigmaPlot 9, as follows: Y = bottom + (top – bottom)(1 – exp[–kx]), where k is the rate constant and t_1/2 is 0.69/k.

General techniques. Western blot and quantitative reverse transcription-PCR (qRT-PCR) analyses were carried out as described previously (3, 4). Cdc42-Rac interactive binding domain (CRIB) pull-down experiments were performed as described previously (24), using the CRIB domain of S. cerevisiae Ste20 (amino acids 304 to 346) cloned into the pGEX 6P-2 plasmid (a gift from D. McCusker). Exponentially growing cells were lysed in GPLB (20 mM Tris-Cl, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 5 mM glycerol phosphate, 1 mM dithiothreitol, Boehringer Mannheim protease inhibitor cocktail, 0.5% NP-40) by glass bead agitation in a Ribolyser. The supernatant collected after centrifugation at 5,000 x g was incubated with CRIB-glutathione S-transferase-glutathione-agarose for 1 h at 4°C. After three successive washes with GPLB, proteins were eluted with Laemmli buffer and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Top Abstract Introduction Materials and Methods Results Discussion References

 
C. albicans Rac1 identification. C. albicans ORF19.6237 was identified by a BLAST search (1) using the human Rac1 sequence. A protein sequence alignment of this ORF with those of Rac1 proteins from different organisms, such as Y. lipolytica, Ustilago maydis, Caenorhabditis elegans, Drosophila melanogaster, and human, is illustrated in Fig. 1. ORF19.6237 is highly identical to the Rac1 proteins in all these species, with an overall sequence identity, shown in gray, of approximately 60%. ORF19.6237 has the conserved amino acid sequence TKXD at positions 116 to 119 (shifted one amino acid due to an insertion of aspartic acid at position 108) which is found in all Rac proteins, in contrast to the conserved sequence TQXD found in all Cdc42 proteins (7), including C. albicans Cdc42. Furthermore, ORF19.6237 has additional Rac-specific residues, including a tryptophan at position 56 which is critical for Rac signaling specificity (8), in contrast with the phenylalanine which is found at this position in Cdc42 proteins. These invariant Rac-specific amino acid residues indicate that the C. albicans ORF19.6237 protein is part of the Rac family of GTPases, and henceforth it will be referred to as Rac1. The most striking difference in C. albicans Rac1 is a stretch of 43 amino acids unique to this protein. In C. albicans, as in other organisms, Rac1 and Cdc42 are also highly similar to one another (57% overall sequence identity), suggesting that these two G proteins may have similar functions.

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FIG. 1. Comparison of Rac1 proteins from different species. C. albicans (Ca), Y. lipolytica (Yl), U. maydis (Um), C. elegans (Ce), D. melanogaster (Dm), and human (Hs) proteins were compared. Gray boxes indicate sequence identity.

Rac1 is not required for C. albicans viability. To determine the importance of Rac1 in C. albicans development, we successively disrupted the two copies of RAC1. RAC1 gene disruption was verified by PCR (Fig. 2A) and by qRT-PCR (Fig. 2B). RAC1 mRNA was undetectable in rac1/rac1 mutant cells. In wild-type cells, RAC1 mRNA levels were threefold lower than those of CDC42. Dilutions of rac1/rac1 mutant cells spotted on YEPD medium (Fig. 2C) showed that growth of this RAC1 deletion strain was similar to that of the wild-type cells, and furthermore, doubling times in liquid medium were indistinguishable between rac1/rac1 mutant and wild-type cells. These results indicate that Rac1, in contrast to Cdc42 (3, 17, 21), is not required for C. albicans viability. We next examined the actin cytoskeleton in the rac1/rac1 mutant strain, and Fig. 2D shows no significant differences in actin patch and cable organization between this deletion mutant and the wild type. These results indicate that Rac1 is not required for actin organization in C. albicans.

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FIG. 2. Characterization of C. albicans RAC1 deletion mutant. (A) RAC1 deletion construction. PCR analysis was performed with total genomic DNAs from wild-type (PY82) and rac1/RAC1 (PY256) and rac1/rac1 (PY191) mutant strains, using the primer pair RAC1.P5 and RAC1.P6. For the rac1/rac1 mutant strain, no 0.8-kb RAC1 band was detected. (B) RAC1 is not expressed in the deletion mutant. The levels of RAC1 and CDC42 transcripts in the wild-type and rac1/rac1 mutant strains were quantified by qRT-PCR, using RAC1.P11 and -P12 or CDC42.P3 and -P4 primers. Values are relative to the actin message, amplified with previously described primers (4). (C) Rac1 is not required for viability. Dilutions of the indicated strains were spotted on YEPD plates and grown for 3 days. (D) Rac1 is not required for actin cytoskeleton organization. Differential interference contrast and deconvoluted fluorescence images of the indicated strains stained with Alexa-phalloidin are shown. Fluorescence images are maximum intensity projections of z sections (11 to 15 by 0.1 µm).

C. albicans Rac1 is a bona fide G protein. We constructed a fusion of Rac1 with green fluorescent protein (GFP), which migrates with an apparent M_w larger than that of the corresponding GFP-Cdc42 fusion (Fig. 3A), in agreement with the difference in ORF sizes. G proteins cycle between an inactive GDP-bound state and an active GTP-bound state. We measured the amount of active Rac1 by using a CRIB pull-down assay (24); the CRIB domain only binds to Rac1-GTP or Cdc42-GTP. Figure 3B illustrates the results of such a pull-down experiment for strains expressing GFP-Rac1 (WT) and its dominant active (G12V) or dominant negative (T17N) version. The level of Rac1 in the GTP-bound form determined from three independent experiments was 0.4% ± 0.1% of the total Rac1 input. The level of Cdc42-GTP determined similarly was 0.5% ± 0.2% of the total Cdc42 input (data not shown). These results indicate that Rac1 binds GTP to the same extent as Cdc42 in C. albicans.

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FIG. 3. C. albicans Rac1 binds GTP. (A) Immunoblot analysis of Cdc42 and Rac1. Extracts of GFP-Cdc42 (PY196)- and GFP-Rac1 (PY205)-expressing cells were analyzed by SDS-PAGE, followed by immunoblotting and probing with anti-GFP polyclonal sera. (B) CRIB pull-down assay with C. albicans Rac1. Clarified cell lysates of cells expressing GFP-Rac1, dominant active GFP-Rac1(G12V) (PY209), dominant negative GFP-Rac1(T17N) (PY212), or no GFP-Rac1 (PY191) were incubated with CRIB-glutathione S-transferase-glutathione-agarose resin. Eluted proteins were separated by SDS-PAGE and analyzed as in panel A. Lanes 5 to 8 were loaded with 1% of total Rac1.

Rac1 is critical for filamentation in embedded media. Different environments can trigger C. albicans filamentous growth. To investigate the role of Rac1 in such responses, we examined the growth of the RAC1 deletion mutant in response to different stimuli. Cdc42 is required for C. albicans invasive hyphal growth in response to serum (3, 23). In contrast, Rac1 is not necessary for such responses, since the RAC1 deletion mutant invaded fetal calf serum (FCS)-containing agar to a similar extent as a wild-type strain (Fig. 4A, upper panel) and formed hyphae (95%) after 3 h of incubation at 37°C in liquid medium (Fig. 4A, lower panel), similar to wild-type cells. Furthermore, rac1/rac1 mutant cells responded to two other inducers of filamentous growth, i.e., Spider medium and N-acetylglucosamine, similar to wild-type cells (data not shown).

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FIG. 4. Rac1 is essential for embedded medium-induced but not serum-induced filamentous growth. (A) Serum-induced filamentous growth. Wild-type (PY82) and rac1/rac1 mutant (PY191) strains were grown in the presence of YEPD and FCS. Images are of colonies grown for 5 days (upper panel) and of cells incubated for 3 h at 37°C in liquid medium (lower panel). (B) Embedded medium-induced filamentous growth. Images of colonies of the wild type, the rac1/rac1 mutant strain, the rac1/rac1 mutant strain complemented with RAC1 (PY275), and the cdc42/PMETCDC42 mutant strain (PY123) were taken after 6 days of growth at 25°C embedded in agar containing YEP plus sucrose. (C) Percentages of filamentous colonies in the strains illustrated in panel B and the rac1/rac1 PADHGFPRAC1 mutant strain (PY205). Averages of four to six experiments (n = 200 colonies each) are shown, with standard deviations indicated.

Mutants such as the efg1/efg1 mutant, which is defective in serum-induced responses (16), can nevertheless respond by filamentous growth when embedded in solid media (9). Similarly, we observed that cdc42/PMETCDC42 mutants, which are unable to form hyphae in YEPD-FCS medium (3), could respond to YEP-sucrose embedded medium by filamentous growth (Fig. 4B, upper panel), although the filaments were not as long as those in the wild-type strain. Strikingly, we did not observe filamentation of rac1/rac1 mutant colonies in such an embedded medium (Fig. 4B, lower panel). This defect was indeed due to the RAC1 deletion, as it was complemented by the reintroduction of RAC1. Wild-type cells, cdc42 mutant cells, and rac1/rac1 mutant cells with reintroduced RAC1 all formed >90% filamentous colonies, whereas rac1/rac1 mutant cells formed <5% filamentous colonies (Fig. 4C). Furthermore, rac1/rac1 mutant cells which overexpressed GFP-Rac1 (via the ADH promoter) also formed >90% filamentous colonies, indicating that GFP-Rac1 is functional. Together, these results indicate that Rac1 is required for filamentation under embedded conditions.

Rac1 and Cdc42 have different dynamics at the membrane. In C. albicans, Cdc42 localizes to the plasma membrane, where it is clustered at the tips of growing buds or hyphae (11) (Fig. 5A). Similarly, Rac1 is localized to the plasma membrane during both vegetative and serum-induced hyphal growth (Fig. 5A), yet its membrane localization is more homogenous than that of Cdc42, with little to no enrichment at the tips of either buds, hyphae, or septa. In order to examine the dynamics of these two G proteins, we carried out FRAP experiments using functional GFP fusions (Fig. 4C and 6A and B) in strains in which these fusions were the sole copies of the respective G protein. The fluorescence at the tips of either buds or hyphae of cells expressing GFP-Rac1 and GFP-Cdc42 was photobleached (Fig. 5A), and the recovery of fluorescence over time was monitored (Fig. 5B). The average FRAP t_1/2 values were 5.1 ± 1.5 s (n = 17) and 3.4 ± 1.7 s (n = 12) for Cdc42 in buds and hyphae, respectively. A similar value of 5 s has been observed for Cdc42 in budding S. cerevisiae cells (25). Strikingly, the FRAP t_1/2 of Rac1 was approximately three to four times faster than that of Cdc42, with average FRAP t_1/2 values of 1.4 ± 0.3 s (n = 13) for buds and 1.3 ± 0.4 s (n = 14) for hyphae. Furthermore, the FRAP t_1/2 values for Rac1 were similar irrespective of the location of photobleaching (cell body or hyphae). These results indicate that compared to Cdc42, membrane-associated Rac1 is highly dynamic, and they suggest that these two G proteins have different interactions at the membrane.

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FIG. 5. Rac1 and Cdc42 localize to the plasma membrane with different dynamics. (A) Rac1 localizes uniformly to the plasma membrane in yeast and hyphal cells. rac1/rac1 mutant cells expressing GFP-Rac1 (PY205) and cdc42/PMETCDC42 mutant cells expressing GFP-Cdc42 (PY196) were used for FRAP analyses. PY196 cells were grown in medium containing 2.5 mM methionine and cysteine to repress the expression of endogenous CDC42. Confocal microscopy images of budding cells or cells undergoing hyphal growth in response to serum at 37°C for 2 h were taken prior to and subsequent to (10 to 100 ms) photobleaching and after fluorescence recovery. The arrowheads indicate the bleached areas. (B) Single-phase exponential curve fits of fluorescence recovery after photobleaching intensities. Average intensities of bleached areas normalized for photobleaching during image acquisition are shown. Arrowheads indicate the times of the images illustrated in panel A.

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FIG. 6. Rac1 and Cdc42 cannot substitute for one another in C. albicans growth. (A) Rac1 overexpression does not complement for viability in cdc42/PMETCDC42 mutant strains. A dilution series of the cdc42/PMETCDC42 mutant strain overexpressing GFP-Cdc42 (PY196) or GFP-Rac1 (PY225) was spotted on plates of the indicated media and incubated for 3 days. (B) Rac1 overexpression does not complement the Cdc42 requirement for serum-induced hyphal growth. Images of exponentially growing cells from the wild-type strain (PY82), the cdc42/PMETCDC42 mutant strain (PY123), and the cdc42/PMETCDC42 mutant strain overexpressing Cdc42 (PY115), GFP-Cdc42, Rac1 (PY317), or GFP-Rac1 were taken after growth in the absence (YEPD alone) or presence of FCS (50% YEPD, 50% FCS) for 3 h.

Rac1 overexpression does not complement Cdc42 function. Rac1 and Cdc42 have both complementary and overlapping functions in different organisms. To investigate if these two G proteins have overlapping functions in C. albicans, we initially examined the effect of Rac1 overexpression in cdc42/PMETCDC42 mutant cells with respect to cell viability and serum-induced hyphal growth. As illustrated in Fig. 6A, GFP-Rac1 overexpression did not restore growth to cdc42/PMETCDC42 mutant cells on a medium which represses CDC42 expression, indicating that Rac1 overexpression cannot complement Cdc42 function for viability. In contrast, overexpression of GFP-Cdc42 enabled cdc42/PMETCDC42 mutants to grow on a repressive medium, indicating that this GFP fusion is functional for viability. When grown in YEPD medium, which contains a low methionine level (semipermissive conditions) (3), cdc42/PMETCDC42 mutant cells appeared larger than wild-type cells (Fig. 6B). The overexpression of Cdc42 or GFP-Cdc42 in the cdc42/PMETCDC42 mutant cells resulted in cells that were similar in size to wild-type cells and restored serum-induced hyphal growth, indicating that GFP-Cdc42 is functional. Overexpression of Rac1 or GFP-Rac1 in the cdc42/PMETCDC42 mutant strain resulted in cells which appeared larger than cdc42/PMETCDC42 mutant cells. Wild-type cells were not affected by the overexpression of Rac1 or GFP-Rac1 in rich medium; however, we observed some heterogeneity in cell size (data not shown). Nonetheless, these cells formed hyphae in the presence of serum. Rac1 or GFP-Rac1 overexpression did not restore serum-induced hyphal growth in cdc42/PMETCDC42 mutant cells, and instead, these cells were substantially larger than those not overexpressing Rac1 (Fig. 6B). These results suggest that Rac1 can interfere with polarized growth, in particular during hyphal growth, in cells with reduced levels of Cdc42. Perhaps Rac1 titrates out a Cdc42 effector, for example, a CRIB domain-containing protein. Conversely, overexpression of CDC42 did not restore embedded filamentous growth in rac1/rac1 mutant cells (data not shown). Taken together, these results indicate that Cdc42 and Rac1 do not have overlapping functions, but rather that these two G proteins play different roles in C. albicans development.

Top Abstract Introduction Materials and Methods Results Discussion References

 
We have identified and characterized Rac1 of C. albicans. This G protein is highly similar to Rac1 proteins in other organisms and to Cdc42. However, in contrast to Cdc42, Rac1 is not necessary for viability in C. albicans. Strikingly, rac1/rac1 mutant cells do not form filamentous colonies when embedded in an agar matrix, yet these cells form hyphal filaments in response to serum. Rac1 localized to the plasma membrane, albeit with a more uniform distribution than that of Cdc42, which is found clustered at bud and hyphal tips, as previously reported (11). Using FRAP methods, we showed that Rac1 has different dynamics at the plasma membrane than Cdc42, suggesting that these two proteins have different interactions at the membrane. Furthermore, overexpression studies indicated that Cdc42 and Rac1 do not have overlapping functions, as each protein was unable to substitute for the function of the other. Together, our results indicate that these two G proteins have distinct functions in C. albicans.

Surprisingly, Rac1, which is critical for actin cytoskeleton organization in metazoans, is not required for actin organization in C. albicans. The requirement for Rac1 in actin cytoskeleton organization of different fungi varies, as Rac1 in both Y. lipolytica and C. neoformans is not necessary for actin organization (12, 22), in contrast to the case of P. marneffei, where Rac1 is important for this process (5). C. albicans Rac1 can bind GTP, as shown by CRIB pull-down experiments. Because Rac1 in the GTP-bound state can bind the S. cerevisiae Ste20 CRIB domain, it is likely that this G protein, similar to Cdc42, binds effector proteins in vivo.

Different environmental signals, such as N-acetylglucosamine, an alkaline extracellular pH, serum, and entrapment in a semisolid matrix, can trigger filamentous growth of the human pathogen C. albicans (26). In response to serum, signaling via a cyclic AMP-protein kinase A pathway results in the induction of Efg1-dependent hypha-specific genes such as HGC1 (28). Cdc42 has been demonstrated to be specifically required for the maintenance of Efg1-dependent gene induction (4, 23). Both cdc42 mutants and strains expressing reduced levels of this G protein undergo vegetative growth yet do not undergo invasive filamentous growth in response to serum (3, 4, 17, 21, 23). However, we showed here that such strains with a reduced level of Cdc42 form filamentous colonies when embedded in a semisolid agar matrix. The phenotype of cells with ectopic Cdc42 expression is similar to that of efg1/efg1 cph1/cph1 mutants, which do not form hyphae in response to serum (16) but, nonetheless, form filamentous colonies in embedded media (9), further supporting the hypothesis that Cdc42 and Efg1 signal via the same pathway.

In response to entrapment in a semisolid matrix, the Czf1 transcription factor is required (6). CZF1 was isolated in a screen for genes whose ectopic expression stimulated filamentous colonies when cells were grown under embedded conditions (6). czf1/czf1 mutant cells exhibited a delay in filamentous growth under embedded conditions, and this defect was further enhanced by the deletion of CPH1 from this czf1/czf1 mutant strain. In contrast, both czf1/czf1 mutant cells and czf1/czf1 cph1/cph1 mutant cells formed filaments similar to those of the wild type in Spider medium, serum-containing medium, and N-acetylglucosamine-containing medium. rac1/rac1 mutant cells exhibited similar responses to filamentation inducers, i.e., embedded conditions, Spider medium, serum, and N-acetylglucosamine, suggesting that Rac1 may signal via Czf1, raising the possibility that the two very similar G proteins Cdc42 and Rac1 function in different signal transduction pathways. How these two similar G proteins function in different developmental states in C. albicans is currently under investigation.

 
This work was supported by the CNRS, Fondation pour la Recherche Médicale-BNP-Paribas, and La Ligue Contre le Cancer. The ABI Prism 7000 instrument and the Zeiss LSM 510 META confocal microscope were financed in part by Association pour la Recherche sur le Cancer grants 7696 and 7830.

We thank J. Hopkins for qRT-PCR analyses, C. Matthews for aid with microscopy, C. Favard for aid with FRAP analysis, and D. McCusker for the CRIB construct.

 
* Corresponding author. Mailing address: Institute of Signaling, Developmental Biology, and Cancer, CNRS UMR 6543, Université de Nice, Faculté des Sciences-Parc Valrose, 06108 Nice Cedex 2, France. Phone: 33-492076464. Fax: 33-492076466. E-mail: mbassila{at}unice.fr.

Top Abstract Introduction Materials and Methods Results Discussion References

 

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Eukaryotic Cell, February 2006, p. 321-329, Vol. 5, No. 2
1535-9778/06/$08.00+0     doi:10.1128/EC.5.2.321-329.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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