Identification in the Ancient Protist Giardia lamblia of Two Eukaryotic Translation Initiation Factor 4E Homologues with Distinctive Functions
Eukaryotic Cell Li and Wang
4: 948
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Files in this Data Supplement:
- Supplemental file 1
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Fig. S1, Purification of recombinant Giardia eIF4Es
Legend for Supplementary Fig. S1. Purification of recombinant Giardia eIF4Es. 6-His tagged eIF4E1 and eIF4E2 were over expressed in transformed E. coli and purified using Ni-NTA beads under denaturing conditions. Purified samples of eIF4E1 (A) and eIF4E2 (B) were analyzed in SDS-PAGE and stained with Coomassie Blue for indication of purity.
PowerPoint presentation, 91K.
- Supplemental file 2
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Fig. S2, MALDI-TOF analysis of the m7GTP binding protein from
Giardia
Supplementary Fig. S2. MALDI-TOF analysis of the m7GTP binding protein from Giardia. The cap-binding protein identified in Fig. 2A was cut off the gel, digested with trypsin and subject to MALDI-TOF analysis. (A) The profile of peptides in MALDI-TOF; (B) Identified peptide sequences (underlined) that cover 46% of the eIF4E2 sequence.
PowerPoint presentation, 62K.
