Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Escalante, R.
Right arrow Articles by Loomis, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Escalante, R.
Right arrow Articles by Loomis, W. F.

 Previous Article

Eukaryotic Cell, April 2004, p. 564-566, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.564-566.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Genes Dependent on the MADS Box Transcription Factor SrfA in Dictyostelium discoideum Development{dagger}

Ricardo Escalante,1 Negin Iranfar,2 Leandro Sastre,1* and William F. Loomis2

Instituto de Investigaciones Biomédicas CSIC/UAM, 28029 Madrid, Spain,1 Cell and Developmental Biology, Division of Biology, University of California at San Diego, La Jolla, California 920932

Received 13 November 2003/ Accepted 12 January 2004


   ABSTRACT
Top
 Abstract
Text
 References
 
Analysis of microarrays containing 6,345 Dictyostelium discoideum genes has identified 21 whose expression is dependent on the MADS box transcription factor SrfA. In wild-type cells, all of these genes are induced late in development. At least four of them are necessary for proper spore differentiation, stability, and/or germination.


   TEXT
Top
 Abstract
 Text
References
 
Dictyostelium discoideum strains carrying null mutations in the srfA gene form abnormal spores that do not resist adverse conditions (4). srfA codes for a protein homologue to serum response factor transcription factors that bind to the minor grove of DNA through a conserved domain, the MADS box (13, 17). Ultrastructural analyses of srfA– spores have shown that actin rods are initiated but do not elongate as in wild-type spores and subsequently disaggregate. The spore coats of srfA– spores are initially indistinguishable from those of wild-type spores but become shredded with time (7). These structural changes are accompanied by reduced expression of spore-specific genes, such as spiA (3, 4). Altogether, these data suggest that srfA is necessary for the late steps of spore differentiation.

Microarrays containing 6,345 D. discoideum cDNA clones (9), including 690 previously characterized developmental genes and 5,655 cDNA clones from the Japanese EST Project (12), were used to identify genes dependent on SrfA for their expression. With Trizol reagent (Gibco BRL), RNAs were isolated from wild-type (AX4) and srfA– (IIB100, derived from AX4) strains at 2-h intervals throughout development on filters (16) and compared to time-averaged RNA from wild-type cells, as previously described (9). Temporal changes for each gene were analyzed in an Axon Genepix 4000B scanner with GeneSpring software from Silicon Genetics. The total Cy3 signal was normalized to the total Cy5 signal after background subtraction to allow independent slides to be compared. The Cy3/Cy5 ratios of individual genes were then calculated. Each sample was hybridized to two or more microarrays, and each developmental time course was repeated at least twice. A list of the genes and mean values used for subsequent analyses are available at http://www.biology.ucsd.edu/loomis-cgi/microarray/paper2.html (see Tables S1 and S2 in the supplemental material).

Genes that were expressed at lower levels in the mutant cells than in wild-type cells were all induced after 20 h of development. Therefore, RNAs from the two last developmental stages of each strain (22 and 24 h for wild-type cells and 26 and 28 h for srfA– cells, due to a slight delay in culmination observed in the mutant strain [6]) were directly compared to each other. Microarrays were simultaneously hybridized with Cy3 and Cy5 probes generated from wild-type and srfA– strains, respectively. Thirty genes showed a more-than-threefold higher signal level with wild-type samples than with mutant samples and were considered candidates for SrfA-dependent genes. Microarray data are publically available at http://www.biology.ucsd.edu/loomis-cgi/microarray/srfA_paper.html (see Table S3 in the supplemental material).

The pattern of expression of the potential SrfA-dependent genes was further analyzed by Northern blot hybridization as previously described (5). Twenty-two genes were confirmed to be SrfA dependent (Fig. 1 and 2). The cDNA clones have been grouped according to their temporal pattern of expression. The expression of the first group of 11 genes, shown in Fig. 1 and Table 1, is detected exclusively during culmination in wild-type cells. The expression of the second group of 11 genes, shown in Fig. 2 and Table 1, is detected at low levels at earlier stages and induced at high levels during culmination. This late induction was not observed in srfA– strains. As a control, we analyzed several genes that are expressed late in development but did not give higher signals for wild-type samples than for srfA– samples on the microarrays, including the well-characterized prespore-specific gene pspA (1, 2). Northern blot analyses confirmed these results (data not shown). We also determined the cell type specificity of these genes by interrogating the published microarray data for separated prestalk and prespore cells (10, 11). Ten of the SrfA-dependent genes are preferentially enriched in prespore cells and spores, while three are preferentially expressed in prestalk and stalk cells (Table 1).



View larger version (71K):
[in this window]
[in a new window]
  		FIG. 1. Developmental expression of SrfA-dependent genes expressed only late in development. Total RNA was isolated from wild-type (AX4) or srfA-null cells at the indicated times of development (hours). After electrophoretic separation and transfer to nylon membranes, each blot was hybridized to a PCR probe corresponding to the cDNA indicated on the left. The right column indicates the ratio of the signal on microarrays from AX4 cells at 22 and 24 h of development to that from srfA– cells at 26 and 28 h of development.

 


View larger version (61K):
[in this window]
[in a new window]
  		FIG. 2. Developmental expression of SrfA-dependent genes that are expressed early in development and up-regulated late in development. Total RNA was isolated from wild-type (AX4) or srfA-null cells at the indicated times of development (hours). Northern blots were hybridized to the cDNA probe indicated on the left. The right column indicates the ratio of the signal on microarrays from AX4 cells at 22 and 24 h of development to that from srfA– cells at 26 and 28 h of development for each gene.

 

View this table:
[in this window]
[in a new window]
  		TABLE 1. Possible functions of the proteins encoded by SrfA-dependent genesa

 
Nine of the SrfA-dependent genes encode known Dictyostelium proteins (present in the Preliminary Directory of Dictyostelium Genes [http://dicty.sdsc.edu/annot-020303.html]), and the others show significant similarity to known proteins in other organisms (Table 1). Two of the cDNA clones, SSK268 and SLJ453, coded for nonoverlapping regions of the same gene. Three other genes (sigB, sigC, and sigD), previously recognized from a subtractive library to be SrfA dependent (3) but not represented on the microarrays, were included in Table 1 for completeness. Mutational analyses have shown that catB, plcD, cofB, and spiA are each necessary for normal spore maturation or germination. catB codes for catalase B and mutant spores have been shown to be abnormally sensitive to H2O2 (8). plcD codes for phospholipase C{delta}, which is required for regulation of spore germination (18). cofB codes for cofilin B, which associates with the actin rods in mature spores (15). Absence of actin rods results in round spores with very low viability. Spores deficient in SpiA show decreased viability under submerged conditions (14). Null mutations in malA, sigB, sigC, or sigD cause no apparent defects in spores (3). The other SrfA-dependent genes can be clustered on the basis of putative function of their closest homologs (Table 1). Possible functions include stress responses, actin cytoskeleton organization, metabolic regulation, prespore vesicle fusion, and spore coat stability.

In summary, a total of 24 SrfA-dependent genes have been identified, all of which are expressed late in development. No genes differentially expressed in slug or mid-culmination structures were found, even if srfA is expressed at these developmental stages (6). In addition, these studies have uncovered a novel program of gene expression that is activated late in Dictyostelium development. Thirty-nine genes were found that increased their expression at least threefold between 22 and 24 h of development. A significant proportion of these genes are dependent on SrfA for their expression and might be involved in many of the processes required for terminal spore differentiation.


   ACKNOWLEDGMENTS
 
We are indebted to the Japanese EST Project for supplying inserts of cDNA clones and the BioGEM facility of the University of California at San Diego for arraying them. Annotation of the microarrayed cDNAs benefited from the whole genome sequences generated by the Dictyostelium Sequencing Consortium at the Baylor Sequencing Center, Houston, Tex.; the Institute of Biochemistry, Cologne, Germany; the Institute of Molecular Biotechnology, Jena, Germany; and the Welcome Trust Sanger Institute, Hinxton, England.

This work was supported by grants from the National Institutes of Health (GM62350) and the Spanish Ministerio de Ciencia y Tecnología (BMC2002-01501).


   FOOTNOTES
 
* Corresponding author. Mailing address: Instituto de Investigaciones Biomédicas, CSIC/UAM, Arturo Duperier, 4, 28029 Madrid, Spain. Phone: 34 91 585 4437. Fax: 34 91 585 4401. E-mail: lsastre{at}iib.uam.es. Back

{dagger} Supplemental material for this article may be found at http://ec.asm.org/. Back


   REFERENCES
Top
 Abstract
 Text
 References
 

  1. Barklis, E., and H. F. Lodish. 1983. Regulation of Dictyostelium discoideum mRNAs specific for prespore or prestalk cells. Cell 32:1139-1148.[CrossRef][Medline]
  2. Early, A. E., J. G. Williams, H. E. Meyer, S. B. Por, E. Smith, K. L. Williams, and A. A. Gooley. 1988. Structural characterization of Dictyostelium discoideum prespore-specific gene D19 and of its product, cell surface glycoprotein PsA. Mol. Cell. Biol. 8:3458-3466.[Abstract/Free Full Text]
  3. Escalante, R., N. Moreno, and L. Sastre. 2003. Identification of Dictyostelium discoideum developmentally regulated genes whose expression is dependent on the MADS box transcription factor SrfA. Eukaryot. Cell 2:1327-1335.[Abstract/Free Full Text]
  4. Escalante, R., and L. Sastre. 1998. A serum response factor homolog is required for spore differentiation in Dictyostelium. Development 125:3801-3808.[Abstract]
  5. Escalante, R., and L. Sastre. 1993. Similar alternative splicing events generate two sarcoplasmic or endoplasmic reticulum Ca-ATPase isoforms in the crustacean Artemia franciscana and in vertebrates. J. Biol. Chem. 268:14090-14095.[Abstract/Free Full Text]
  6. Escalante, R., J. J. Vicente, N. Moreno, and L. Sastre. 2001. The MADS-box gene srfA is expressed in a complex pattern under the control of alternative promoters and is essential for different aspects of Dictyostelium development. Dev. Biol. 235:314-329.[CrossRef][Medline]
  7. Escalante, R., Y. Yamada, D. Cotter, L. Sastre, and M. Sameshima. 2004. The MADS-box transcription factor SrfA is required for actin cytoskeleton organization and spore coat stability during Dictyostelium sporulation. Mech. Dev. 121:51-56.[CrossRef][Medline]
  8. Garcia, M. X., H. Alexander, D. Mahadeo, D. A. Cotter, and S. Alexander. 2003. The Dictyostelium discoideum prespore-specific catalase B functions to control late development and to protect spore viability. Biochim. Biophys. Acta 1641:55-64.[Medline]
  9. Iranfar, N., D. Fuller, and W. F. Loomis. 2003. Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum. Eukaryot. Cell 2:664-670.[Abstract/Free Full Text]
  10. Iranfar, N., D. Fuller, R. Sasik, T. Hwa, M. Laub, and W. F. Loomis. 2001. Expression patterns of cell-type-specific genes in Dictyostelium. Mol. Biol. Cell 12:2590-2600.[Abstract/Free Full Text]
  11. Maeda, M., H. Sakamoto, N. Iranfar, D. Fuller, T. Maruo, S. Ogihara, T. Morio, H. Urushihara, Y. Tanaka, and W. F. Loomis. 2003. Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses. Eukaryot. Cell 2:627-637.[Abstract/Free Full Text]
  12. Morio, T., H. Urushihara, T. Saito, Y. Ugawa, H. Mizuno, M. Yoshida, R. Yoshino, B. N. Mitra, M. Pi, T. Sato, K. Takemoto, H. Yasukawa, J. Williams, M. Maeda, I. Takeuchi, H. Ochiai, and Y. Tanaka. 1998. The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res. 5:335-340.[Abstract]
  13. Pellegrini, L., S. Tan, and T. J. Richmond. 1995. Structure of serum response factor core bound to DNA. Nature 376:490-498.[CrossRef][Medline]
  14. Richardson, D. L., and W. F. Loomis. 1992. Disruption of the sporulation-specific gene spiA in Dictyostelium discoideum leads to spore instability. Genes Dev. 6:1058-1070.[Abstract/Free Full Text]
  15. Sameshima, M., Y. Kishi, M. Osumi, D. Mahadeo, and D. A. Cotter. 2000. Novel actin cytoskeleton: actin tubules. Cell Struct. Funct. 25:291-295.[CrossRef][Medline]
  16. Shaulsky, G., and W. F. Loomis. 1993. Cell type regulation in response to expression of ricin-A in Dictyostelium. Dev. Biol. 160:85-98.[CrossRef][Medline]
  17. Treisman, R. 1995. DNA-binding proteins. Inside the MADS box. Nature 376:468-469.[CrossRef][Medline]
  18. van Dijken, P., and P. J. M. van Haastert. 2001. Phospholipase C{delta} regulates germination of Dictyostelium spores. BMC Cell Biol. 2:25-31.[CrossRef][Medline]

Eukaryotic Cell, April 2004, p. 564-566, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.564-566.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:


This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Escalante, R.
Right arrow Articles by Loomis, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Escalante, R.
Right arrow Articles by Loomis, W. F.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS