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Eukaryotic Cell, July 2009, p. 968-976, Vol. 8, No. 7
1535-9778/09/$08.00+0     doi:10.1128/EC.00353-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Increased [PSI+] Appearance by Fusion of Rnq1 with the Prion Domain of Sup35 in Saccharomyces cerevisiae {triangledown}

Young-Jun Choe, Yangkyun Ryu, Hyun-Jin Kim, and Yeong-Jae Seok*

Laboratory of Macromolecular Interactions, Department of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea

Received 28 October 2008/ Accepted 21 April 2009

During propagation, yeast prions show a strict sequence preference that confers the specificity of prion assembly. Although propagations of [PSI+] and [RNQ+] are independent of each other, the appearance of [PSI+] is facilitated by the presence of [RNQ+]. To explain the [RNQ+] effect on the appearance of [PSI+], the cross-seeding model was suggested, in which Rnq1 aggregates act as imperfect templates for Sup35 aggregation. If cross-seeding events take place in the cytoplasm of yeast cells, the collision frequency between Rnq1 aggregates and Sup35 will affect the appearance of [PSI+]. In this study, to address whether cross-seeding occurs in vivo, a new [PSI+] induction method was developed that exploits a protein fusion between the prion domain of Sup35 (NM) and Rnq1. This fusion protein successfully joins preexisting Rnq1 aggregates, which should result in the localization of NM around the Rnq1 aggregates and hence in an increased collision frequency between NM and Rnq1 aggregates. The appearance of [PSI+] could be induced very efficiently, even with a low expression level of the fusion protein. This study supports the occurrence of in vivo cross-seeding between Sup35 and Rnq1 and provides a new tool that can be used to dissect the mechanism of the de novo appearance of prions.

* Corresponding author. Mailing address: Laboratory of Macromolecular Interactions, Department of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea. Phone: 82-2-880-8827. Fax: 82-2-888-4911. E-mail: yjseok{at}snu.ac.kr

{triangledown} Published ahead of print on 1 May 2009.

Eukaryotic Cell, July 2009, p. 968-976, Vol. 8, No. 7
1535-9778/09/$08.00+0     doi:10.1128/EC.00353-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ross, C. D., McCarty, B. R., Hamilton, M., Ben-Hur, A., Ross, E. D. (2009). A Promiscuous Prion: Efficient Induction of [URE3] Prion Formation by Heterologous Prion Domains. Genetics 183: 929-940 [Abstract] [Full Text]  


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