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Eukaryotic Cell, June 2009, p. 916-919, Vol. 8, No. 6
1535-9778/09/$08.00+0     doi:10.1128/EC.00041-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Using Morpholinos for Gene Knockdown in Giardia intestinalis{triangledown} ,{dagger}

Meredith L. Carpenter and W. Zacheus Cande*

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720

Received 2 February 2009/ Accepted 2 April 2009

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720. Phone: (510) 642-1669. Fax: (510) 643-6791. E-mail: zcande{at}berkeley.edu

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, June 2009, p. 916-919, Vol. 8, No. 6
1535-9778/09/$08.00+0     doi:10.1128/EC.00041-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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