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Eukaryotic Cell, April 2009, p. 550-559, Vol. 8, No. 4
1535-9778/09/$08.00+0     doi:10.1128/EC.00350-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Correlation between Biofilm Formation and the Hypoxic Response in Candida parapsilosis{triangledown} ,{dagger}

Tristan Rossignol,1,2,{ddagger} Chen Ding,2,{ddagger} Alessandro Guida,3 Christophe d'Enfert,1 Desmond G. Higgins,3 and Geraldine Butler2*

Institut Pasteur, Unité Biologie et Pathogénicité Fongiques, INRA USC2019, 25 rue du Docteur Roux, 75724 Paris, France,1 UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland,2 UCD School of Medicine and Medical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland3

Received 20 October 2008/ Accepted 8 January 2009

The ability of Candida parapsilosis to form biofilms on indwelling medical devices is correlated with virulence. To identify genes that are important for biofilm formation, we used arrays representing approximately 4,000 open reading frames (ORFs) to compare the transcriptional profile of biofilm cells growing in a microfermentor under continuous flow conditions with that of cells in planktonic culture. The expression of genes involved in fatty acid and ergosterol metabolism and in glycolysis, is upregulated in biofilms. The transcriptional profile of C. parapsilosis biofilm cells resembles that of Candida albicans cells grown under hypoxic conditions. We therefore subsequently used whole-genome arrays (representing 5,900 ORFs) to determine the hypoxic response of C. parapsilosis and showed that the levels of expression of genes involved in the ergosterol and glycolytic pathways, together with several cell wall genes, are increased. Our results indicate that there is substantial overlap between the hypoxic responses of C. parapsilosis and C. albicans and that this may be important for biofilm development. Knocking out an ortholog of the cell wall gene RBT1, whose expression is induced both in biofilms and under conditions of hypoxia in C. parapsilosis, reduces biofilm development.

* Corresponding author. Mailing address: UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland. Phone: 353-1-7166885. Fax: 353-1-7166456. E-mail: Geraldine.Butler{at}ucd.ie

{triangledown} Published ahead of print on 16 January 2009.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} T.R. and C.D. contributed equally to this work.

Eukaryotic Cell, April 2009, p. 550-559, Vol. 8, No. 4
1535-9778/09/$08.00+0     doi:10.1128/EC.00350-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stichternoth, C., Ernst, J. F. (2009). Hypoxic Adaptation by Efg1 Regulates Biofilm Formation by Candida albicans. Appl. Environ. Microbiol. 75: 3663-3672 [Abstract] [Full Text]  


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