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Eukaryotic Cell, February 2009, p. 170-180, Vol. 8, No. 2
1535-9778/09/$08.00+0     doi:10.1128/EC.00330-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Global Gene Expression Analysis during Germination in the Chytridiomycete Blastocladiella emersonii{triangledown} ,{dagger}

Silvia M. Salem-Izacc, Tie Koide, Ricardo Z. N. Vêncio,# and Suely L. Gomes*

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil

Received 29 September 2008/ Accepted 8 December 2008

Blastocladiella emersonii is an aquatic fungus of the Chytridiomycete class. During germination, the zoospore, a motile nongrowing cell, goes through a cascade of morphological changes that culminates with its differentiation into the germling cell, capable of coenocytic vegetative growth. Transcriptome analyses of B. emersonii cells were carried out during germination induced under various environmental conditions. Microarray data analyzing 3,563 distinct B. emersonii genes revealed that 26% of them are differentially expressed during germination in nutrient medium at at least one of the time points investigated. Over 500 genes are upregulated during the time course of germination under those conditions, most being related to cell growth, including genes involved in protein biosynthesis, DNA transcription, energetic metabolism, carbohydrate and oligopeptide transport, and cell cycle control. On the other hand, several transcripts stored in the zoospores are downregulated during germination in nutrient medium, such as genes involved in signal transduction, amino acid transport, and chromosome organization. In addition, germination induced in the presence of nutrients was compared with that triggered either by adenine or potassium ions in inorganic salt solution. Several genes involved in cell growth, induced during germination in nutrient medium, do not show increased expression when B. emersonii zoospores germinate in inorganic solution, suggesting that nutrients exert a positive effect on gene transcription. The transcriptome data also revealed that most genes involved in cell signaling show the same expression pattern irrespective of the initial germination stimulus.

* Corresponding author. Mailing address: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, SP, Brazil. Phone: 55-11-3091-3826. Fax: 55-11-3091-2186. E-mail: sulgomes{at}iq.usp.br

{triangledown} Published ahead of print on 19 December 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

# Present address: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

Eukaryotic Cell, February 2009, p. 170-180, Vol. 8, No. 2
1535-9778/09/$08.00+0     doi:10.1128/EC.00330-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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