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Eukaryotic Cell, September 2008, p. 1433-1440, Vol. 7, No. 9
1535-9778/08/$08.00+0     doi:10.1128/EC.00119-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Schizosaccharomyces pombe Noc3 Is Essential for Ribosome Biogenesis and Cell Division but Not DNA Replication{triangledown}

Christopher R. Houchens,1* Audrey Perreault,2 François Bachand,2 and Thomas J. Kelly1

Laboratory of Regulation of DNA Replication, Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021,1 Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, Canada2

Received 4 April 2008/ Accepted 23 June 2008

The initiation of eukaryotic DNA replication is preceded by the assembly of prereplication complexes (pre-RCs) at chromosomal origins of DNA replication. Pre-RC assembly requires the essential DNA replication proteins ORC, Cdc6, and Cdt1 to load the MCM DNA helicase onto chromatin. Saccharomyces cerevisiae Noc3 (ScNoc3), an evolutionarily conserved protein originally implicated in 60S ribosomal subunit trafficking, has been proposed to be an essential regulator of DNA replication that plays a direct role during pre-RC formation in budding yeast. We have cloned Schizosaccharomyces pombe noc3+ (Spnoc3+), the S. pombe homolog of the budding yeast ScNOC3 gene, and functionally characterized the requirement for the SpNoc3 protein during ribosome biogenesis, cell cycle progression, and DNA replication in fission yeast. We showed that fission yeast SpNoc3 is a functional homolog of budding yeast ScNoc3 that is essential for cell viability and ribosome biogenesis. We also showed that SpNoc3 is required for the normal completion of cell division in fission yeast. However, in contrast to the proposal that ScNoc3 plays an essential role during DNA replication in budding yeast, we demonstrated that fission yeast cells do enter and complete S phase in the absence of SpNoc3, suggesting that SpNoc3 is not essential for DNA replication in fission yeast.

* Corresponding author. Mailing address: Memorial Sloan-Kettering Cancer Center, Rockefeller Research Laboratories 609, 430 East 67th Street, New York, NY 10021. Phone: (212) 639-3264. Fax: (646) 422-2189. E-mail: houchenc{at}mskcc.org

{triangledown} Published ahead of print on 7 July 2008.

Eukaryotic Cell, September 2008, p. 1433-1440, Vol. 7, No. 9
1535-9778/08/$08.00+0     doi:10.1128/EC.00119-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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